Description

Poly-L-Ornithine Hydrochloride is a synthetic, positively charged polyamino acid derived from the non-proteinogenic amino acid ornithine. This compound is essential for creating stable, positively charged surfaces that promote the adhesion of cells, particularly neurons and other anchorage-dependent cell types. It is a critical reagent in life science research, biotechnology, and advanced materials science. Key users include R&D laboratories, pharmaceutical developers, and manufacturers of cell culture products and diagnostic substrates.

Application

• Cell Culture Substrate Coating: Widely used to coat glass or plastic surfaces to enhance the attachment and growth of primary neurons, endothelial cells, and other sensitive cell lines.
• Neuroscience Research: Serves as a standard substrate for culturing hippocampal, cortical, and other neuronal cells to study neurodevelopment, neurotoxicity, and synaptic function.
• Biomaterial and Tissue Engineering: Employed to functionalize scaffolds and materials to improve cell-material interactions for regenerative medicine applications.
• Diagnostic Device Manufacturing: Used as an adhesion layer in the production of certain biosensors and microarray slides to immobilize biomolecules.
• Polyelectrolyte Multilayer (PEM) Assembly: Acts as a cationic layer in the layer-by-layer (LbL) fabrication of thin films for drug delivery systems and smart coatings.
• Virus and Gene Delivery Studies: Utilized to complex with nucleic acids or modify surfaces to enhance transfection efficiency in vector development research.

Basic Information

Product Name	Poly-L-Ornithine Hydrochloride
CAS No.	26982-21-8
Molecular Formula	(C5H12N2O2 • HCl)n
Molecular Weight	132.62 (monomer unit, free base) / Varies by chain length (n)
Synonyms	Poly(ornithine) hydrochloride; PLO; Poly-L-Ornithine HBr (related salt); Polyornithine Hydrobromide; L-Ornithine homopolymer hydrochloride; Poly(Orn) • HCl; (S)-Poly(2,5-diaminopentanoic acid) hydrochloride; NSC 77625
EINECS	Contact for details

Quality Control

Our Poly-L-Ornithine Hydrochloride is manufactured under strict quality management systems. Each batch is tested to ensure consistent molecular weight distribution, purity, and biological performance. A comprehensive Certificate of Analysis (COA) is provided with each shipment, detailing key parameters such as assay, endotoxin levels, sterility (where applicable), and functional cell culture testing. We support compliance with relevant guidelines for research and in-vitro diagnostic use.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at 2-8°C. The product is hygroscopic; allow the vial to equilibrate to room temperature before opening to prevent moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder or lyophilized solid
Identification (IR)	Conforms to structure
Assay (Nitrogen)	≥ 95.0%
Molecular Weight Range	15,000 - 70,000 Da (Typical, other ranges available)
Solubility	Clear, colorless solution in water (1% w/v)
pH (1% Solution)	5.0 - 7.0
Endotoxin	< 1.0 EU/mg (for cell culture grade)
Biological Activity (Cell Culture)	Supports neuronal cell adhesion and neurite outgrowth

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.