Description

Affi-Gel 731 is a specialized boronate affinity chromatography medium designed for the selective capture and purification of cis-diol-containing biomolecules. This product is critical for achieving high-purity separations in complex biological mixtures, offering superior binding capacity and specificity. It is an essential tool for researchers and process developers in the biotechnology and pharmaceutical industries, particularly for the purification of glycoproteins, nucleotides, carbohydrates, and catecholamines.

Application

• Purification of glycoproteins, glycopeptides, and antibodies with glycosylated regions.
• Isolation and analysis of ribonucleotides, oligonucleotides, and RNA species.
• Separation of carbohydrates, sugars, and other cis-diol-containing metabolites in analytical and preparative chromatography.
• Capture of catecholamines and related neurotransmitters for neurological research and diagnostic applications.
• Sample preparation and clean-up in proteomics and metabolomics workflows prior to mass spectrometry analysis.
• Development and scale-up of downstream bioprocessing steps for biologics and vaccine production.
• Immobilization of enzymes or other biomolecules via their cis-diol groups for biocatalysis or biosensor applications.

Basic Information

Product Name	Affi-Gel 731
CAS No.	78564-78-0
Molecular Formula	Contact for details
Molecular Weight	N/A (Chromatography Medium)
Synonyms	Boronate Affinity Gel; 3-Aminophenylboronic acid agarose; PBA-agarose; m-Aminophenylboronic acid cross-linked agarose; Affi-Gel® 731; Boronate ester gel; cis-Diol specific affinity medium; Bio-Rad Affi-Gel 731; Boronic acid functionalized agarose
EINECS	Contact for details

Quality Control

Our Affi-Gel 731 is manufactured under a rigorous quality management system. Each lot is tested to ensure consistent performance characteristics, including binding capacity, ligand density, particle size distribution, and flow properties. Certificates of Analysis (COA) detailing these specifications are available upon request to support your quality and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). The product is supplied as a suspension in a storage solution; do not allow the gel to dry out. For long-term storage, ensure the container is sealed to prevent evaporation and microbial contamination.

Specification

Item	Specification
Matrix	Cross-linked 6% agarose beads
Ligand	m-Aminophenylboronic acid
Ligand Density	~10-12 µmol/mL settled gel
Binding Capacity	≥ 2 mg adenosine/mL gel (typical)
Particle Size	45-165 µm (wet)
Exclusion Limit	~4x10⁷ Daltons
pH Stability	2-12 (long term); 4-10 (operational)
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.