Description

Lac Repressor Fragment 33-38 CAS NO 78228-88-3 is a synthetic peptide fragment derived from the DNA-binding domain of the Lac repressor protein. This compound is a critical research tool for studying protein-DNA interactions, gene regulation mechanisms, and molecular recognition events. It is primarily utilized by biochemists, molecular biologists, and pharmaceutical researchers in academic, biotechnology, and R&D settings for fundamental research and assay development.

Application

• Biochemical Research: Fundamental studies on the mechanism of gene repression and the Lac operon system.
• Protein-DNA Interaction Assays: Used as a specific binding substrate in gel-shift assays (EMSA), surface plasmon resonance (SPR), and isothermal titration calorimetry (ITC) to characterize binding kinetics and affinity.
• Molecular Biology Reagent: Serves as a competitive inhibitor or control in experiments designed to probe DNA-binding specificity of transcription factors.
• Drug Discovery Screening: Potential target fragment for identifying small molecules that modulate transcription factor activity in high-throughput screening campaigns.
• Structural Biology: Crystallization and NMR studies to determine the three-dimensional structure of protein-DNA complexes.
• Educational & Training Kits: Component in advanced laboratory courses for teaching principles of molecular genetics and biochemistry.

Basic Information

Product Name	Lac Repressor Fragment 33-38
CAS No.	78228-88-3
Molecular Formula	C30H53N11O9
Molecular Weight	687.82 g/mol
Synonyms	Lac Repressor (33-38); LacI Fragment 33-38; H-Lys-Leu-Ala-Pro-Val-Asp-OH; KLAPVD; DNA-binding domain peptide of Lac repressor (residues 33-38); Lac Repressor headpiece fragment (33-38); LacI (33-38) peptide
EINECS	Contact for details

Quality Control

Our Lac Repressor Fragment 33-38 is produced under stringent conditions to ensure batch-to-batch consistency and high purity suitable for sensitive research applications. Each lot undergoes comprehensive analytical characterization. A detailed Certificate of Analysis (COA) is provided, which includes data for purity (HPLC), molecular weight confirmation (MS), and peptide content. We adhere to ISO 9001 quality management principles in our manufacturing processes.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a freezer. This product is hygroscopic (moisture-sensitive). Allow the vial to reach room temperature in a desiccator before opening to prevent condensation and moisture uptake. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (HPLC/MS)	Retention time and mass consistent with standard
Purity (HPLC)	≥ 95%
Molecular Weight	687.82 ± 1.0 Da (by MS)
Peptide Content	≥ 80% (by amino acid analysis)
Solubility	Soluble in water, dilute aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.