Description

4-Bromo-1-Oxyl-2,2,5,5-Tetramethyl-δ3-Pyrroline-3-Carboxylic Acid is a specialized nitroxide radical compound, also known as a spin label, with the CAS number 78033-69-9. It serves as a critical molecular probe for investigating structure, dynamics, and interactions in complex biological and chemical systems using Electron Paramagnetic Resonance (EPR) spectroscopy. This high-purity intermediate is essential for researchers and manufacturers in advanced fields such as structural biology, polymer science, and materials chemistry who require reliable, well-characterized spin labels for their studies.

Application

• Site-Directed Spin Labeling (SDSL) in proteins and nucleic acids for studying conformational changes and macromolecular interactions.
• Polymer and Materials Science as a radical initiator or a probe for monitoring polymerization kinetics, cross-linking density, and material mobility.
• EPR Spectroscopy Standards and reference materials for instrument calibration and method development.
• Oxidation Catalysis and organic synthesis as a stable nitroxide radical catalyst or mediator.
• Biophysical Research for probing membrane fluidity, protein folding, and distance measurements via Double Electron-Electron Resonance (DEER).
• Pharmaceutical Research in the development of novel therapeutics where understanding molecular binding and dynamics is crucial.

Basic Information

Product Name	4-Bromo-1-Oxyl-2,2,5,5-Tetramethyl-δ3-Pyrroline-3-Carboxylic Acid
CAS No.	78033-69-9
Molecular Formula	C10H15BrNO3
Molecular Weight	277.14 g/mol
Synonyms	4-Bromo-2,2,5,5-tetramethyl-δ3-pyrroline-3-carboxylic acid-1-oxyl; 4-Bromo-PROXYL carboxylic acid; 3-Carboxy-PROXYL bromide; 4-Bromo-2,2,5,5-tetramethyl-3-pyrroline-3-carboxylic acid-1-oxyl; 4-Bromo-3-carboxy-2,2,5,5-tetramethylpyrrolidine-1-oxyl; Br-PROXYL-COOH; Spin label Br-PROXYL; 4-Bromo-1-oxy-2,2,5,5-tetramethyl-3-pyrroline-3-carboxylic acid
EINECS	Contact for details

Quality Control

Our 4-Bromo-1-Oxyl-2,2,5,5-Tetramethyl-δ3-Pyrroline-3-Carboxylic Acid is manufactured under strict quality protocols to ensure high purity and batch-to-batch consistency suitable for sensitive research applications. Each lot is characterized by advanced analytical techniques including HPLC, NMR, and EPR spectroscopy. Certificates of Analysis (COA) detailing purity, identity, and radical concentration are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This compound is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to air, moisture, or light may affect stability and radical activity. For long-term storage, consider storing under an inert atmosphere.

Specification

Item	Specification
Appearance	Orange to red crystalline powder
Identification (IR)	Conforms to structure
Identification (HPLC)	Conforms to reference
Purity (HPLC)	≥ 97.0%
Assay (EPR)	≥ 95.0% radical content
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.