Description

Alveolysin CAS NO 77751-18-9 is a highly purified, pore-forming cytolysin derived from *Streptococcus pneumoniae*. This specialized reagent is critical for research and development in microbiology and immunology, enabling the controlled lysis of eukaryotic cells. It is primarily utilized by biotechnology, pharmaceutical, and academic research institutions for applications in vaccine development, host-pathogen interaction studies, and membrane protein research.

Application

• Vaccine Research & Development: Used as a tool antigen or in challenge studies for pneumococcal vaccine efficacy testing.
• Host-Pathogen Interaction Studies: Essential for investigating the mechanisms of pneumococcal virulence and cellular toxicity.
• Cell Lysis & Membrane Protein Extraction: Employed for the selective permeabilization of eukaryotic cell membranes to isolate intracellular components or study pore formation.
• Immunology Assays: Serves as a key antigen in ELISA, Western blot, and other immunoassays to detect specific antibodies.
• Biochemical Research: Used in studies of cholesterol-dependent cytolysins (CDCs) and their role in bacterial pathogenesis.
• Diagnostic Development: A potential component in the development of diagnostic kits for detecting pneumococcal infections.

Basic Information

Product Name	Alveolysin
CAS No.	77751-18-9
Molecular Formula	C5H9NO3S
Molecular Weight	163.19 g/mol
Synonyms	Pneumolysin; PLY; Streptococcus pneumoniae Pneumolysin; Thiol-activated cytolysin; Cholesterol-dependent cytolysin (CDC); Pneumococcal hemolysin; S. pneumoniae toxin
EINECS	Contact for details

Quality Control

Our Alveolysin is produced under stringent quality management systems. Each batch is subjected to comprehensive analytical testing including identity confirmation by mass spectrometry, purity assessment by HPLC, and functional activity assays to ensure consistent biological potency. Certificates of Analysis (COA) detailing purity, endotoxin levels, and specific activity are provided with every shipment to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure containers are sealed under anhydrous conditions.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Specific Activity	≥ 1.0 x 106 HU/mg
Endotoxin Level (LAL)	< 1.0 EU/µg
Protein Content (BCA)	≥ 90% of dry weight
Solubility	Soluble in aqueous buffer
Moisture Content (KF)	≤ 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.