Description

6-(7-β-Galactosylcoumarin-3-Carboxamido)Hexylamine is a specialized synthetic derivative of coumarin, functionalized with a galactosyl group and a hexylamine linker. This compound is primarily valued as a highly sensitive fluorogenic substrate for the detection and quantification of β-galactosidase enzyme activity. It is an essential tool for researchers and quality control professionals in the biotechnology, pharmaceutical, and diagnostic industries, where precise enzymatic assays are critical for development and testing.

Application

• Enzymatic Assay Substrate: A fluorogenic probe for detecting β-galactosidase activity in reporter gene assays (e.g., lacZ gene expression).
• Cell Biology & Microbiology Research: Used to study gene expression patterns, cell lineage, and microbial identification.
• Diagnostic Kit Development: A key component in the formulation of diagnostic kits for enzymatic deficiencies and related disorders.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify modulators of β-galactosidase activity.
• Biochemical Research: Used for kinetic studies and enzyme characterization in vitro.
• Quality Control Testing: Applied in biomanufacturing to monitor and validate purification processes involving β-galactosidase tags.

Basic Information

Product Name	6-(7-β-Galactosylcoumarin-3-Carboxamido)Hexylamine
CAS No.	77750-09-5
Molecular Formula	C26H36N2O11
Molecular Weight	552.57 g/mol
Synonyms	6-[(7-β-D-Galactopyranosyloxy)-2-oxo-2H-chromene-3-carboxamido]hexylamine; 6-[(7-β-D-Galactopyranosyloxy)coumarin-3-carboxamido]hexylamine; β-Galactosidase Substrate (Fluorogenic); C12FDG (6-Chloro-2-hydroxyacridinone) analog; Galactosylcoumarin hexylamine; 7-β-Galactosyl-3-carboxycoumarin hexylamide; Fluorogenic β-Gal substrate; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Our production of 6-(7-β-Galactosylcoumarin-3-Carboxamido)Hexylamine adheres to stringent in-process controls to ensure batch-to-batch consistency and high chemical purity, suitable for sensitive research applications. Each lot is characterized by HPLC, NMR, and mass spectrometry to confirm identity and purity. Certificates of Analysis (COA) with detailed chromatographic data are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This compound is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Retention time corresponds to reference standard
Identification (NMR)	Spectrum consistent with structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.