Description

Isoleucyl-Prolyl-Arginine-4-Nitroanilide is a specialized chromogenic peptide substrate designed for enzymatic activity assays. This compound is critical for researchers and quality control laboratories requiring precise, colorimetric detection of specific protease activity. It is primarily utilized in the pharmaceutical, biotechnology, and diagnostic industries for enzyme kinetics studies, inhibitor screening, and biochemical research.

Application

• Protease Activity Assays: As a chromogenic substrate for the detection and quantification of specific serine proteases, including thrombin and related enzymes.
• Biochemical Research: Used in enzyme kinetics studies to determine kinetic parameters (Km, Vmax) and inhibition constants (Ki).
• Pharmaceutical Development: Employed in high-throughput screening (HTS) platforms to identify and characterize novel protease inhibitors for drug discovery.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for coagulation disorders and other clinical assays.
• Quality Control (QC) Testing: Used to standardize and validate the activity of enzyme preparations in biomanufacturing processes.

Basic Information

Product Name	Isoleucyl-Prolyl-Arginine-4-Nitroanilide
CAS No.	77672-35-6
Molecular Formula	C24H38N10O6
Molecular Weight	562.63 g/mol
Synonyms	Ile-Pro-Arg-pNA; Isoleucine-Proline-Arginine-4-Nitroanilide; IPR-pNA; Chromogenic Substrate for Thrombin; Thrombin Substrate I; H-Ile-Pro-Arg-pNA; Tosyl-Gly-Pro-Arg-p-nitroanilide acetate salt (related); S-2238 (related chromogenic substrate); p-Nitroanilide peptide substrate.
EINECS	Contact for details

Quality Control

Our Isoleucyl-Prolyl-Arginine-4-Nitroanilide is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and related substances is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability and performance.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; slightly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.