Description

Toyopearl Hw55F is a high-performance, hydrophobic interaction chromatography (HIC) resin designed for the purification of biomolecules. This product is critical for achieving high-resolution separation of proteins, peptides, and nucleic acids based on their surface hydrophobicity. It is an essential tool for downstream processing in the biopharmaceutical, vaccine development, and life science research sectors, enabling scalable and reproducible purification workflows.

Application

• Monoclonal Antibody (mAb) Purification: Intermediate purification and polishing steps to remove aggregates, host cell proteins, and process-related impurities.
• Recombinant Protein Purification: Isolation and purification of therapeutic proteins, enzymes, and growth factors from complex fermentation broths.
• Vaccine Production: Downstream processing for the purification of viral vectors, virus-like particles (VLPs), and subunit vaccines.
• Nucleic Acid Separation: Purification of plasmid DNA, mRNA, and other oligonucleotides in gene therapy and vaccine applications.
• Peptide Purification: Resolution of synthetic or recombinant peptides in pharmaceutical and cosmetic ingredient manufacturing.
• Process Development & Scale-Up: Used in laboratory-scale process optimization and seamlessly scalable to pilot and industrial manufacturing columns.

Basic Information

Product Name	Toyopearl Hw55F
CAS No.	77642-25-2
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Hydrophobic Interaction Chromatography Resin HW55F; TOSOH HIC Resin HW55F; Polymeric HIC Medium; Butyl HIC Resin; Phenyl HIC Resin (functional group dependent); Hydrophobic Chromatography Gel; 77642-25-2; HIC Media for Biomolecule Purification
EINECS	Contact for details

Quality Control

Our Toyopearl Hw55F chromatography resin is manufactured under a strict quality management system. Each lot is tested to ensure consistent performance characteristics, including particle size distribution, ligand density, pressure-flow properties, and dynamic binding capacity. A Certificate of Analysis (COA) detailing these specifications is provided with every shipment to guarantee it meets the rigorous demands of cGMP and biopharmaceutical production environments.

Storage

Preserve in a tightly closed container, protected from light. Store the resin slurry in the supplied storage solution (typically 20% ethanol) at a controlled room temperature (15-25°C). Do not freeze. For long-term storage, ensure the container is sealed to prevent evaporation and microbial contamination.

Specification

Item	Specification
Matrix	Hydrophilic Polymeric
Particle Size	55 μm (mean)
Ligand Type	Butyl or Phenyl (specify grade)
Dynamic Binding Capacity*	> 50 mg IgG/mL resin
pH Stability	2 - 13 (long term), 1 - 14 (cleaning)
Maximum Pressure	0.3 MPa
Storage Solution	20% Ethanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.