Description

Pyridine-2-Azo-4-Dimethylaniline Cephalosporin is a specialized chromogenic cephalosporin derivative, primarily utilized as a key biochemical reagent for the detection and study of β-lactamase activity. This compound offers a critical advantage by providing a sensitive and colorimetric method for enzymatic assays, enabling precise microbiological research and diagnostic development. It is an essential tool for pharmaceutical R&D laboratories, academic research institutions, and manufacturers of in-vitro diagnostic kits focused on antibiotic resistance mechanisms.

Application

• β-Lactamase Detection: Serves as a primary chromogenic substrate in biochemical assays to detect and quantify β-lactamase enzyme activity in bacterial cultures.
• Antibiotic Resistance Research: Used in research laboratories to study the mechanisms and kinetics of antibiotic resistance, particularly in Gram-positive and Gram-negative bacteria.
• Diagnostic Kit Manufacturing: A critical raw material in the production of chromogenic culture media and diagnostic test kits for clinical microbiology.
• Pharmaceutical Development: Employed in the screening and development of new β-lactam antibiotics and β-lactamase inhibitors.
• Microbiological Quality Control: Applied in pharmaceutical and food industry QC labs to test for β-lactamase-producing contaminants.
• Enzymatic Studies: Utilized as a model substrate in enzymology to understand substrate specificity and catalytic efficiency.

Basic Information

Product Name	Pyridine-2-Azo-4-Dimethylaniline Cephalosporin
CAS No.	77449-91-3
Molecular Formula	C20H19N7O4S2
Molecular Weight	485.54 g/mol
Synonyms	PADAC; Chromogenic Cephalosporin Substrate; Pyridine-2-Azo-p-Dimethylaniline Cephalosporin; 7-(Thien-2-ylacetamido)-3-[[[2-(4-dimethylaminophenylazo)pyridinium-1-yl]methyl]carbamoylmethyl]-3-cephem-4-carboxylic Acid; Cefazolin Chromogenic Derivative; β-Lactamase Chromogenic Substrate; Nitrocefin Analog; Chromogenic β-Lactam Substrate
EINECS	Contact for details

Quality Control

Our Pyridine-2-Azo-4-Dimethylaniline Cephalosporin is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) with detailed chromatographic data are provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept tightly sealed in a dry environment to prevent moisture absorption, which can affect stability and performance. For long-term storage, consider desiccants.

Specification

Item	Specification
Appearance	Orange to red powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, Methanol; Slightly soluble in water
Functional Activity	Conforms to reference in β-lactamase assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.