Description

Butyloxycarbonyl-Alanyl-Alanyl-Phenylalanine-Isoluminolamide is a specialized synthetic peptide derivative that incorporates an isoluminolamide moiety, making it a valuable chemiluminescent probe. This compound is engineered for high-sensitivity detection and quantification in advanced biochemical assays, offering a stable and efficient alternative to traditional fluorescent labels. It is primarily utilized by research institutions and diagnostic manufacturers in the fields of immunoassay development, enzymatic activity studies, and molecular diagnostics.

Application

• Chemiluminescent Immunoassays (CLIA): Serves as a highly sensitive label for antibodies or antigens in diagnostic test kits for disease markers, hormones, and infectious agents.
• Biosensor Development: Used as a signal-generating component in biosensors for environmental monitoring, food safety testing, and point-of-care diagnostics.
• Enzyme Activity & Inhibition Studies: Acts as a substrate or probe for proteases and other hydrolytic enzymes, enabling real-time kinetic analysis.
• Molecular Biology Research: Employed in nucleic acid hybridization assays and other detection systems requiring amplified luminescent signals.
• Pharmaceutical R&D: Utilized in high-throughput screening (HTS) platforms to identify and characterize new drug candidates.
• Bioconjugation & Labeling: Provides a reactive handle for covalent attachment to proteins, oligonucleotides, or other biomolecules.

Basic Information

Item	Details
Product Name	Butyloxycarbonyl-Alanyl-Alanyl-Phenylalanine-Isoluminolamide
CAS No.	77303-12-9
Molecular Formula	C31H40N6O8
Molecular Weight	624.69 g/mol
Synonyms	Boc-Ala-Ala-Phe-Isoluminolamide; Boc-AAF-Isoluminolamide; t-Butyloxycarbonyl-Ala-Ala-Phe-Isoluminolamide; N-(tert-Butoxycarbonyl)-L-alanyl-L-alanyl-L-phenylalanyl-6-amino-2,3-dihydrophthalazine-1,4-dione; Chemiluminescent Peptide Substrate; CL Peptide Probe
EINECS	Contact for details

Quality Control

Our Butyloxycarbonyl-Alanyl-Alanyl-Phenylalanine-Isoluminolamide is manufactured under controlled conditions to ensure batch-to-batch consistency and high purity, suitable for sensitive research and diagnostic applications. Quality is verified through a comprehensive suite of analytical techniques including HPLC, MS, and NMR. A Certificate of Analysis (COA) detailing purity, identity, and other critical parameters is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.