Description

n-Ethylmaleimide-β-Galactoside is a specialized chemical reagent designed for advanced biochemical and bioconjugation applications. This compound is valued for its role as a glycosidase substrate and its ability to act as a Michael acceptor for selective thiol modification, enabling precise protein and enzyme labeling. It is primarily utilized by researchers and manufacturers in the fields of glycobiology, enzymology, and diagnostic assay development, where reliable, high-purity reagents are critical for experimental success and product consistency.

Application

• Enzymatic Activity Assays: Used as a chromogenic or fluorogenic substrate for detecting and quantifying β-galactosidase activity in research and diagnostic kits.
• Protein Labeling & Conjugation: The n-ethylmaleimide moiety facilitates site-specific covalent attachment to cysteine residues in proteins and peptides for creating bioconjugates.
• Glycobiology Research: Serves as a tool compound for studying glycosidase enzyme kinetics, inhibition, and mechanism of action.
• Diagnostic Reagent Manufacturing: A key intermediate in the synthesis of reagents for clinical chemistry and point-of-care testing devices.
• Cell Biology Studies: Employed in assays to monitor gene expression (e.g., lacZ reporter systems) and cellular processes.
• Biochemical Probe Development: Used to create activity-based probes for imaging and profiling glycosidases in complex biological samples.

Basic Information

Product Name	n-Ethylmaleimide-β-Galactoside
CAS No.	77249-82-2
Molecular Formula	C14H19NO8
Molecular Weight	329.30 g/mol
Synonyms	NEM-β-Gal; N-Ethylmaleimide β-D-galactopyranoside; 5-[(2,3,4,5,6-Pentahydroxyhexyl)oxy]-1-ethyl-1H-pyrrole-2,5-dione; β-Galactosyl-N-ethylmaleimide; Galactose-N-ethylmaleimide conjugate; β-D-Galactopyranoside, 2-(ethylmaleimido)ethyl; NEM-Gal
EINECS	Contact for details

Quality Control

Our n-Ethylmaleimide-β-Galactoside is produced under stringent quality management systems to ensure batch-to-batch consistency and high purity. Each lot undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods. Certificates of Analysis (COA) detailing all specifications are provided to support your quality assurance and regulatory compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain stability.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.