Description

2,5-Diphenyl-3-(p-Diphenyl)Tetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and chromogenic substrate in biochemical assays. Its primary value lies in its ability to form a colored formazan product upon reduction, making it an essential tool for measuring cellular metabolic activity and dehydrogenase enzyme function. This compound is critically needed by researchers and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors for applications such as cell viability and cytotoxicity testing.

Application

• Cell Viability and Proliferation Assays (e.g., MTT Assay Analog): Used to quantify metabolically active cells, a cornerstone of drug discovery and toxicology studies.
• Microbial Viability Testing: Employed in microbiology to assess the metabolic state and growth of bacterial and fungal cultures.
• Dehydrogenase Enzyme Activity Assays: Serves as an electron acceptor in spectrophotometric methods to measure enzymatic activity in clinical and research settings.
• Cytotoxicity Screening: Applied in high-throughput screening to evaluate the toxic effects of chemical compounds on mammalian cell lines.
• Biochemical Research Reagent: Utilized as a sensitive redox indicator in various experimental protocols studying cellular respiration and electron transport chains.
• Seed Viability Testing: Applied in agricultural research to determine the germination potential and metabolic health of seeds.

Basic Information

Product Name	2,5-Diphenyl-3-(p-Diphenyl)Tetrazolium Chloride
CAS No.	77205-78-8
Molecular Formula	C31H23ClN4
Molecular Weight	487.00 g/mol
Synonyms	p-Diphenyltetrazolium Chloride; DPTZ; 3-(4-Diphenylyl)-2,5-diphenyl-2H-tetrazolium chloride; 2,5-Diphenyl-3-(4-diphenylyl)tetrazolium chloride; Tetrazolium Red Chloride; Triphenyl Tetrazolium Chloride derivative; NSC 406050; TTC analog
EINECS	Contact for details

Quality Control

Our 2,5-Diphenyl-3-(p-Diphenyl)Tetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing to ensure high purity and consistent performance for sensitive biochemical applications. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your research and regulatory needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at room temperature (15-25°C). Due to its light-sensitive nature, prolonged exposure to light should be avoided to maintain stability and performance.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%
Heavy Metals	≤ 20 ppm
Solubility	Soluble in DMSO, methanol; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.