Description

Coenzyme A-Sepharose is a high-performance affinity chromatography medium specifically designed for the purification of enzymes and proteins that interact with Coenzyme A (CoA). This product is critical for researchers and process developers requiring high-purity biological molecules for downstream applications. It is primarily utilized in the biotechnology and pharmaceutical industries for enzyme isolation, protein research, and the development of diagnostic assays.

Application

• Enzyme Purification: Affinity purification of CoA-dependent enzymes such as acetyl-CoA synthetase, carnitine palmitoyltransferase, and various acyltransferases.
• Protein Research: Isolation and study of proteins that bind Coenzyme A or its derivatives in metabolic pathways.
• Diagnostic Kit Development: Used in the production of key components for enzymatic diagnostic tests and biosensors.
• Biopharmaceutical Manufacturing: Downstream processing step for purifying therapeutic enzymes or recombinant proteins.
• Metabolomics Studies: Capture and analysis of CoA-bound metabolites from complex biological samples.
• Academic Research: A fundamental tool in biochemistry and molecular biology laboratories for studying cofactor-protein interactions.

Basic Information

Product Name	Coenzyme A-Sepharose
CAS No.	77114-81-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	CoA-Sepharose; Coenzyme A Agarose; CoA-Agarose; Sepharose 4B Coenzyme A; CoA Affinity Gel; Coenzyme A Immobilized on Sepharose 4B; CoA Sepharose 4B; Coenzyme A-Sepharose 4B
EINECS	Contact for details

Quality Control

Our Coenzyme A-Sepharose is manufactured under strict quality protocols. Each batch is tested for ligand density, binding capacity, and matrix stability to ensure consistent and reliable performance. Certificates of Analysis (COA) detailing these parameters are available upon request to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at 2-8°C in the supplied storage solution (typically 20% ethanol). Do not freeze. Keep the suspension homogeneous and avoid drying out the matrix.

Specification

Item	Specification
Matrix	Sepharose 4B
Ligand	Coenzyme A
Ligand Density	1-5 μmol CoA/mL gel (Typical)
Binding Capacity	Contact for details
Particle Size	45-165 μm
Storage Solution	20% Ethanol
pH Stability	3-10 (operational)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.