Description

Calmodulin is a ubiquitous, calcium-binding regulatory protein essential for mediating a vast array of cellular signaling pathways. Its ability to modulate the activity of numerous enzymes and ion channels makes it a critical component in fundamental biological processes. This high-purity product is vital for researchers in biochemistry, molecular biology, and pharmaceutical development, as well as for diagnostic kit manufacturers requiring consistent, reliable protein performance.

Application

• Biochemical Research: Essential reagent for studying calcium-mediated signal transduction, enzyme kinetics (e.g., phosphodiesterase, adenylate cyclase), and protein-protein interactions.
• Pharmaceutical Screening: Used in high-throughput screening (HTS) assays to identify novel drug candidates that target calmodulin-dependent pathways.
• Diagnostic Kit Manufacturing: A key component in enzyme-linked immunosorbent assays (ELISA) and other diagnostic tests designed to detect calcium signaling abnormalities.
• Enzyme Activity Studies: Serves as a critical activator for calmodulin-dependent enzymes in purified systems, enabling precise mechanistic studies.
• Cell Biology & Neuroscience: Utilized in research focused on neuronal function, muscle contraction, cell cycle progression, and immune response, where calcium signaling is paramount.
• Protein Standard & Control: Acts as a well-characterized standard in electrophoresis (SDS-PAGE), chromatography, and mass spectrometry for quality control and method validation.

Basic Information

Item	Detail
Product Name	Calmodulin
CAS No.	77107-46-1
Molecular Formula	C₁₇₀H₂₆₈N₆₈O₅₃S₄ (Approx., varies by species and post-translational modifications)
Molecular Weight	~16.7 kDa (Approx., species-dependent)
Synonyms	CAM; Calmodulin 1; CALM1; CaM; Calcium-Modulated Protein; Phosphodiesterase Activator; Troponin C-like Protein; Calmodulin (Bovine Brain); Calmodulin (Recombinant)
EINECS	Contact for details

Quality Control

Our Calmodulin is produced and tested under stringent conditions to ensure the highest standards of purity and biological activity. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters. We adhere to ISO 9001 quality management principles, and our production environment is suitable for research and diagnostic applications. Certificates of Analysis (COA) are available upon request.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C to -80°C to avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly in a dry environment after each use.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~17 kDa
Purity (HPLC)	≥ 95%
Protein Content (Biuret)	≥ 90%
Biological Activity (PDE Activation Assay)	Fully active
Endotoxin (LAL)	< 1.0 EU/μg
Solubility	Soluble in aqueous buffers

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.