Description

Iodo-α-Bungarotoxin is a potent, radioiodinated derivative of the snake venom neurotoxin, α-bungarotoxin, specifically engineered for high-affinity binding to nicotinic acetylcholine receptors (nAChRs). This compound matters for its critical role as an irreversibly binding antagonist in neuroscience and pharmacology research, enabling precise receptor localization and characterization. It is essential for researchers in neurobiology, toxicology, and drug discovery who require a highly specific, radiolabeled ligand for studying receptor structure, function, and distribution.

Application

• Neuroscience Research: Irreversible labeling and autoradiographic mapping of nicotinic acetylcholine receptors (nAChRs) in brain tissue and neuromuscular junctions.
• Pharmacological Studies: High-affinity competitive binding assays to screen and characterize novel agonists, antagonists, and allosteric modulators of nAChRs.
• Toxicology & Venom Research: Investigation of the molecular mechanisms of snake venom neurotoxins and their interactions with target receptors.
• Drug Discovery Platforms: Utilization in high-throughput screening (HTS) to identify potential therapeutics for neurological disorders like Alzheimer's disease, Parkinson's disease, and myasthenia gravis.
• Receptor Biochemistry: Purification and isolation of nAChR subtypes via affinity chromatography techniques.
• Cellular & Molecular Biology: Studies on receptor trafficking, internalization, and subunit composition in various cell lines and primary cultures.

Basic Information

Product Name	Iodo-α-Bungarotoxin
CAS No.	77097-81-5
Molecular Formula	C285H432N84O86S9 (Approx., for native α-Bungarotoxin)
Molecular Weight	~8000 g/mol (Approx., Monoisotopic)
Synonyms	125I-α-Bungarotoxin; Iodinated α-Bungarotoxin; α-Bungarotoxin, iodinated; Radioiodinated α-Bungarotoxin; I-α-BGTX; Nicotinic Acetylcholine Receptor Antagonist, Iodinated; Bungarus multicinctus Toxin, iodinated derivative
EINECS	Contact for details

Quality Control

Our Iodo-α-Bungarotoxin is produced and handled under stringent conditions to ensure high specific activity and binding affinity. Each batch undergoes rigorous quality testing, including assessment of radiochemical purity and biological activity via receptor binding assays. Certificates of Analysis (COA) detailing specific activity, purity, and buffer composition are available upon request to support your research compliance and reproducibility.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at ≤ -20°C in the buffer solution provided by the manufacturer. Avoid repeated freeze-thaw cycles. Due to its hygroscopic (moisture-sensitive) nature, ensure the container is sealed properly after each use. Handle in accordance with laboratory safety protocols for radioactive materials.

Specification

Item	Specification
Appearance	Clear, colorless to slightly yellow solution
Identification (HPLC)	Conforms to reference standard
Specific Activity	> 2000 Ci/mmol (Contact for exact value)
Radiochemical Purity (TLC/HPLC)	≥ 95%
Biological Activity (Binding Assay)	Fully active, Kd < 1 nM for Torpedo or α7 nAChRs
Buffer	Phosphate Buffered Saline (PBS) with carrier protein
Radioisotope	Iodine-125 (¹²⁵I)
Concentration	~ 0.1 mCi/mL (Contact for details)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.