Description

Octadecyl Silane-Bonded Silica is a high-purity, chromatographic stationary phase material where octadecylsilane (C18) ligands are chemically bonded to a silica gel substrate. This product is essential for achieving high-resolution separations in reversed-phase liquid chromatography (RP-HPLC and UHPLC) due to its excellent hydrophobicity and chemical stability. It is a critical component for analytical laboratories and manufacturers in the pharmaceutical, biotechnology, food safety, and environmental monitoring sectors seeking reliable and reproducible separation performance.

Application

• Reversed-Phase Chromatography (RP-HPLC/UHPLC): Primary stationary phase for the separation of non-polar to moderately polar compounds, including pharmaceuticals, peptides, and natural products.
• Analytical Method Development: Used as the benchmark C18 phase for developing and validating quantitative and qualitative analytical methods in quality control (QC) labs.
• Preparative and Process Chromatography: Scaled-up versions for the purification and isolation of active pharmaceutical ingredients (APIs), fine chemicals, and biomolecules.
• Food and Beverage Analysis: Detection and quantification of additives, pesticides, vitamins, and contaminants.
• Environmental Analysis: Separation of pollutants, polycyclic aromatic hydrocarbons (PAHs), and herbicides in water and soil samples.
• Biopharmaceutical Purification: Used in the polishing steps for monoclonal antibodies, proteins, and other large biomolecules.
• Cosmetic and Personal Care Product Testing: Analysis of preservatives, active ingredients, and potential impurities.

Basic Information

Product Name	Octadecyl Silane-Bonded Silica
CAS No.	77030-95-6
Molecular Formula	Contact for details
Molecular Weight	N/A (Polymeric Material)
Synonyms	Octadecylsilyl Silica Gel; C18 Silica; C18-Bonded Silica; Octadecyl Silica Gel; ODS Silica; Lichrospher RP-18; Zorbax SB-C18; Hypersil ODS; Kromasil C18; Spherisorb ODS1; Nucleosil C18; Reversed-Phase C18 Stationary Phase
EINECS	Contact for details

Quality Control

Our Octadecyl Silane-Bonded Silica is manufactured under strict quality management systems. Each batch undergoes comprehensive testing for critical parameters including carbon load, surface area, pore size, and chromatographic performance to ensure consistency and reliability. Certificates of Analysis (COA) detailing lot-specific results are provided with every shipment. Production can adhere to relevant ISO and cGMP guidelines for pharmaceutical applications upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Keep away from strong acids, strong bases, and excessive moisture to maintain the integrity of the bonded phase. The product is hygroscopic (moisture-sensitive); ensure containers are sealed after use.

Specification

Item	Specification
Appearance	White to off-white free-flowing powder
Particle Size	3 µm, 5 µm, 10 µm (or as specified)
Pore Size	100 Å, 120 Å, 300 Å (or as specified)
Carbon Load (%)	10-22% (Grade dependent)
Surface Area (m²/g)	150-350
Endcapping	Fully endcapped / Partially endcapped
pH Range (Stability)	2.0 - 8.0
Chromatographic Test	Meets performance criteria for tailing factor, plate count, and retention

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.