Description

7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin is a specialized fluorescent dye derivative that combines the photophysical properties of a coumarin scaffold with a reactive maleimide functional group. This compound matters for its ability to form stable covalent bonds with thiol groups, making it an essential tool for labeling and tracking biomolecules in advanced research. It is primarily needed by researchers and manufacturers in the fields of bioconjugation, fluorescence microscopy, and the development of diagnostic assays and sensors.

Application

• Bioconjugation and Protein Labeling: The maleimide group selectively reacts with cysteine thiols in proteins and peptides for fluorescent tagging.
• Fluorescence Microscopy & Imaging: Used as a probe in cellular and tissue imaging due to its strong fluorescence emission.
• Diagnostic Assay Development: Serves as a key component in the design of fluorescent immunoassays and biosensors.
• Polymer & Material Science: Employed to modify polymers and surfaces with fluorescent properties for tracking and analysis.
• Chemical Biology Research: A vital tool for studying biomolecular interactions, localization, and dynamics in live cells.
• Pharmaceutical Analysis: Can be used in analytical methods to detect and quantify thiol-containing drugs or biomolecules.

Basic Information

Product Name	7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin
CAS No.	76877-33-3
Molecular Formula	C25H24N2O4
Molecular Weight	416.47 g/mol
Synonyms	7-(Diethylamino)-3-[4-(2,5-dihydro-2,5-dioxo-1H-pyrrol-1-yl)phenyl]-4-methyl-2H-1-benzopyran-2-one; 4-Methyl-7-diethylaminocoumarin phenylmaleimide; CPM (Coumarin Phenyl Maleimide); Maleimide-coumarin dye; 3-(4-Maleimidophenyl)-7-diethylamino-4-methylcoumarin; Fluorescent maleimide derivative; Thiol-reactive coumarin dye
EINECS	Contact for details

Quality Control

Our production of 7-Diethylamino-3-(4'-Maleimidylphenyl)-4-Methylcoumarin adheres to stringent quality protocols to ensure batch-to-batch consistency and high purity for research applications. All materials are characterized by advanced analytical techniques including HPLC, NMR, and mass spectrometry. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a freezer at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to minimize oxidation and hydrolysis. Allow the container to reach room temperature in a desiccator before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (HPLC)	Conforms to reference standard
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 0.5%
Single Impurity (HPLC)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.