Description

Arginine-4-Nitroanilide CAS NO 76847-45-5 is a specialized chromogenic substrate widely used in enzymatic assays and biochemical research. This high-purity compound is critical for the accurate detection and quantification of trypsin-like proteases, including thrombin and plasmin. It serves as an essential reagent for researchers and quality control laboratories in the pharmaceutical, diagnostic, and life science sectors, enabling precise measurement of enzymatic activity.

Application

• Enzymatic Substrate for Protease Assays: Primary substrate for the colorimetric determination of trypsin, thrombin, and other arginine-specific serine proteases.
• Biochemical Research & Development: Used in kinetic studies, enzyme characterization, and inhibitor screening in academic and industrial R&D settings.
• Diagnostic Reagent Manufacturing: A key component in the formulation of diagnostic kits for coagulation disorders and other clinical assays.
• Pharmaceutical Quality Control: Employed in potency testing and stability studies of biopharmaceuticals derived from or targeting protease pathways.
• Proteomics & Cell Biology: Facilitates the study of protease function and regulation in complex biological systems and signaling pathways.

Basic Information

Product Name	Arginine-4-Nitroanilide
CAS No.	76847-45-5
Molecular Formula	C12H17N7O4
Molecular Weight	323.31 g/mol
Synonyms	L-Arginine 4-nitroanilide; Arg-pNA; Bz-Arg-pNA; Nα-Benzoyl-L-arginine 4-nitroanilide; Benzoyl-L-arginine p-nitroanilide; BAPNA; Bz-Arg-pNA hydrochloride (salt form); Substrate for trypsin
EINECS	Contact for details

Quality Control

Our Arginine-4-Nitroanilide is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive assays. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation. A Certificate of Analysis (COA) detailing all specifications is provided with every shipment to support your quality and regulatory requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive); ensure the container is kept tightly sealed in a low-humidity environment to maintain stability and prevent degradation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Residue on Ignition	≤ 0.1%
Solubility	Clear, yellow solution in DMSO

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.