Description

Acutase CAS NO 75922-86-0 is a specialized enzymatic preparation designed for high-precision cell dissociation in research and bioprocessing applications. Its primary value lies in its gentle yet effective action, which is critical for maintaining high cell viability and integrity during primary cell isolation and subculture. This product is essential for biotechnology, pharmaceutical development, and advanced life science research sectors where reliable and consistent cell harvesting is paramount.

Application

• Primary Cell Isolation: Gentle dissociation of tissues for the establishment of primary cell cultures.
• Stem Cell Research: Detachment of pluripotent and mesenchymal stem cells while preserving their undifferentiated state and functionality.
• Bioprocessing & Cell Therapy: Scalable harvesting of adherent cells (e.g., CHO, HEK293) in vaccine production and therapeutic cell manufacturing workflows.
• Cancer Research: Isolation of tumor cells from tissue specimens for in vitro studies and drug sensitivity testing.
• Regenerative Medicine: Preparation of single-cell suspensions for tissue engineering and 3D bioprinting applications.
• Neuroscience: Dissociation of neural tissues for the culture of neurons, astrocytes, and other CNS-derived cells.

Basic Information

Product Name	Acutase
CAS No.	75922-86-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Accutase; Accutase™; Acutase Cell Detachment Solution; Cell Dissociation Solution; Enzymatic Cell Detachment Reagent; Tissue Dissociation Enzyme; Multicomponent Protease Blend
EINECS	Contact for details

Quality Control

Our Acutase is manufactured under stringent quality management systems to ensure batch-to-batch consistency and performance reliability. Each lot is tested for enzymatic activity, sterility, and the absence of mycoplasma and endotoxins to meet the rigorous demands of cell-based applications. A comprehensive Certificate of Analysis (COA) detailing purity, specific activity, and performance in standardized cell culture assays is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -15°C to -25°C. Avoid repeated freeze-thaw cycles. Thawed aliquots can be stored at 2°C to 8°C for short-term use.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Protein Concentration	Contact for details
Specific Activity	Defined by cell detachment efficiency
Sterility (Membrane Filtration)	Sterile
Mycoplasma	Not Detected
Endotoxin Level	< 1.0 EU/mL
pH	6.5 - 7.5
Osmolality	280 - 320 mOsm/kg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.