Description

H-Leu-Onp Hydrochloride is a protected amino acid derivative, specifically the p-nitrophenyl ester of L-leucine hydrochloride. This compound is a critical building block in solid-phase peptide synthesis (SPPS), enabling the controlled, sequential addition of amino acids. It is essential for researchers and manufacturers in the pharmaceutical and biotechnology sectors who require high-purity intermediates for producing peptides, peptide hormones, and active pharmaceutical ingredients (APIs).

Application

• Solid-Phase Peptide Synthesis (SPPS): As an activated ester for the efficient coupling of the L-leucine residue.
• Pharmaceutical Intermediates: Key raw material in the synthesis of therapeutic peptides and complex organic molecules.
• Biochemical Research: Used in laboratory-scale synthesis for studying protein structure, function, and peptide-based drug discovery.
• Custom Peptide Production: Enables the manufacture of bespoke peptides for diagnostic, research, and development purposes.
• Protecting Group Chemistry: The Onp (p-nitrophenyl) group acts as a protective moiety that can be removed under specific conditions.

Basic Information

Product Name	H-Leu-Onp Hydrochloride
CAS No.	75691-76-8
Molecular Formula	C12H16N2O4•HCl
Molecular Weight	288.73 g/mol
Synonyms	L-Leucine p-nitrophenyl ester hydrochloride; H-Leu-ONp•HCl; N-(tert-Butoxycarbonyl)-L-leucine 4-nitrophenyl ester hydrochloride; Boc-Leu-ONp hydrochloride; (S)-2-((tert-Butoxycarbonyl)amino)-4-methylpentanoic acid 4-nitrophenyl ester hydrochloride; Leucine, N-[(1,1-dimethylethoxy)carbonyl]-, 4-nitrophenyl ester, hydrochloride; Peptide synthesis building block Leu-Onp HCl
EINECS	Contact for details

Quality Control

Our H-Leu-Onp Hydrochloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by IR and NMR spectroscopy, to ensure it meets the high standards required for sensitive synthetic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and must be kept in a desiccated environment to maintain stability and purity. The container should be kept tightly sealed when not in use.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Specific Rotation	-15° to -25° (c=1 in DMF)
Water Content (KF)	≤ 1.0%
Heavy Metals	≤ 20 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.