Description

Uridine 5'-Monophosphate Synthase is a crucial enzyme in the pyrimidine nucleotide biosynthesis pathway, responsible for catalyzing the conversion of orotidine 5'-monophosphate (OMP) to uridine 5'-monophosphate (UMP). This compound is essential for research and development in biochemistry, molecular biology, and pharmaceutical sciences, where understanding nucleotide metabolism is key. It is primarily utilized by academic researchers, diagnostic kit manufacturers, and biotechnology companies focused on enzyme kinetics, metabolic pathway analysis, and drug discovery targeting nucleotide synthesis.

Application

• Biochemical Research: Fundamental studies on pyrimidine biosynthesis, enzyme mechanisms, and metabolic regulation.
• Pharmaceutical Development: Target enzyme for screening potential inhibitors in anticancer and antimicrobial drug discovery programs.
• Diagnostic Reagent Manufacturing: A key component in enzymatic assay kits used for clinical diagnostics and research to measure UMP synthase activity or related metabolites.
• Enzyme Kinetics & Characterization: Used to determine kinetic parameters (Km, Vmax) and study the effects of mutations or modulators on enzyme function.
• Metabolic Engineering: Research tool in synthetic biology for optimizing nucleotide production pathways in microbial systems.
• Educational Purposes: Used in advanced university laboratory courses to demonstrate principles of enzymology and nucleotide metabolism.

Basic Information

Product Name	Uridine 5'-Monophosphate Synthase
CAS No.	74870-74-9
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	UMP Synthase; Orotidine-5'-phosphate decarboxylase/Orotidine-5'-phosphate pyrophosphorylase; UMPS; Orotidine 5'-monophosphate decarboxylase; Orotidylic acid decarboxylase; Orotidine-5'-phosphate decarboxylase; OPRT/ODC bifunctional protein; Pyr5,6
EINECS	Contact for details

Quality Control

Our Uridine 5'-Monophosphate Synthase is produced and handled under stringent quality management protocols. Each batch undergoes rigorous analytical testing to ensure high specific activity, purity, and stability. Quality parameters typically assessed include protein concentration, specific activity (units/mg), and absence of contaminating nucleases or proteases. A comprehensive Certificate of Analysis (COA) detailing purity, activity, and storage buffer composition is provided with every shipment to guarantee traceability and compliance with your research specifications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent moisture absorption and loss of activity. Store in a dedicated freezer for biochemicals.

Specification

Item	Specification
Appearance	Clear to slightly opalescent solution
Protein Concentration	≥ 1.0 mg/mL
Specific Activity	≥ 50 units/mg protein
Purity (SDS-PAGE)	≥ 90%
Buffer Composition	50 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 50% Glycerol, pH 7.5
Storage Temperature	-20°C

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.