Description

5'-Guanylylimidodiphosphate Trisodium Salt is a high-purity, non-hydrolyzable analog of GTP (guanosine triphosphate) widely used in biochemical research. This compound is critical for studying G-protein coupled receptor (GPCR) signaling pathways and other GTPase-dependent processes due to its resistance to hydrolysis. It is an essential reagent for researchers and manufacturers in the pharmaceutical, biotechnology, and life science research sectors, particularly for enzyme kinetics studies, signal transduction assays, and diagnostic kit development.

Application

• G-protein Activation Studies: Used as a stable GTP analog to activate and study heterotrimeric G-proteins and small GTPases like Ras and Rho.
• Biochemical Assay Development: A key component in high-throughput screening (HTS) assays for drug discovery targeting GPCRs and nucleotide-binding proteins.
• Enzyme Kinetics Research: Employed to investigate the mechanisms of GTP-binding enzymes, including elongation factors and kinases, without interference from hydrolysis.
• Diagnostic Manufacturing: Utilized in the production of diagnostic kits for cellular signaling and metabolic pathway analysis.
• Cell Signaling & Transduction: Essential for in vitro studies of intracellular signal cascades that rely on GTP/GDP cycling.
• Academic & Industrial Research: A fundamental tool for basic research in molecular biology, cell biology, and structural biology.

Basic Information

Product Name	5'-Guanylylimidodiphosphate Trisodium Salt
CAS No.	74812-64-9
Molecular Formula	C10H13N6O13P3Na3
Molecular Weight	603.10 g/mol
Synonyms	GMP-PNP trisodium salt; Guanylyl imidodiphosphate trisodium salt; 5'-Guanylyl imidodiphosphate sodium salt; Gpp(NH)p Na3; Guanosine 5'-[β,γ-imido]triphosphate trisodium salt; GMPPNP; Non-hydrolyzable GTP analog; G-protein activator
EINECS	Contact for details

Quality Control

Our 5'-Guanylylimidodiphosphate Trisodium Salt is manufactured under strict quality systems. Each batch undergoes rigorous analytical testing, including HPLC for purity, NMR for structural confirmation, and mass spectrometry. We provide comprehensive Certificates of Analysis (COA) with each shipment, detailing purity, identity, and impurity profiles to ensure consistency for your critical research and production needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to minimize moisture absorption.

Specification

Item	Specification
Appearance	White to off-white powder or solid
Identification (HPLC)	Conforms to reference standard
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in water, buffer solutions
Biological Activity	Activates standard G-protein assay

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.