Description

Thymidine CAS NO 50-89-5 is a fundamental nucleoside essential for DNA synthesis and cellular metabolism. Its high purity and consistent quality are critical for ensuring reliable results in sensitive research and manufacturing processes. This compound is primarily required by the pharmaceutical, biotechnology, and life science research industries for applications ranging from antiviral drug development to molecular biology.

Application

• Pharmaceutical Intermediate: Key building block in the synthesis of antiviral drugs, including antiretroviral therapies like Zidovudine (AZT).
• Cell Culture & Media Supplement: Essential component in cell culture media, particularly for supporting the growth of certain mutant cell lines (e.g., thymidine kinase-deficient cells).
• Molecular Biology Research: Used in DNA labeling, sequencing, and polymerase chain reaction (PCR) techniques.
• Biochemical Assay Standard: Serves as a reference standard in enzymatic assays for thymidine kinase and other nucleoside-metabolizing enzymes.
• Precursor for Modified Nucleosides: Starting material for the chemical synthesis of various labeled (e.g., tritiated) or modified nucleoside analogs.
• Nutritional Science: Studied for its role in nucleotide metabolism and potential dietary supplementation.

Basic Information

Product Name	Thymidine
CAS No.	50-89-5
Molecular Formula	C10H14N2O5
Molecular Weight	242.23 g/mol
Synonyms	1-(2-Deoxy-β-D-ribofuranosyl)-5-methyluracil; 2'-Deoxythymidine; 5-Methyldeoxyuridine; dT; Deoxythymidine; Thymine deoxyriboside; NSC 21548; D-Thymidine
EINECS	200-070-4

Quality Control

Our Thymidine is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation (IR, NMR), to ensure it meets stringent specifications for research and pharmaceutical use. Certificates of Analysis (COA) detailing all test results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a low-humidity environment to maintain stability.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to reference spectrum
Assay (HPLC)	≥98.0%
Water Content (KF)	≤1.0%
Residue on Ignition	≤0.1%
Heavy Metals	≤10 ppm
Specific Rotation	+30.0° to +34.0° (c=1 in H2O)
Related Substances (HPLC)	Total impurities ≤2.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.