Description

Dodecyl β-D-Cellobioside is a high-purity, non-ionic surfactant and detergent specifically designed for membrane protein research and solubilization. This compound matters for its ability to effectively extract and stabilize integral membrane proteins in their native, functional state without causing denaturation. Researchers and manufacturers in structural biology, biochemistry, and pharmaceutical development need it for applications such as X-ray crystallography, cryo-electron microscopy, and drug discovery assays.

Application

• Membrane Protein Solubilization: Primary agent for extracting integral membrane proteins from lipid bilayers for structural and functional studies.
• Biophysical Characterization: Used to maintain protein stability and monodispersity in techniques like Surface Plasmon Resonance (SPR), Isothermal Titration Calorimetry (ITC), and Analytical Ultracentrifugation (AUC).
• Structural Biology: Critical detergent for preparing samples for X-ray crystallography and single-particle cryo-electron microscopy (cryo-EM).
• Drug Discovery: Enables the study of membrane protein targets (e.g., GPCRs, ion channels) in high-throughput screening and binding assays.
• Protein Purification: Incorporated into chromatography buffers to purify membrane proteins while preserving activity.
• Reconstitution Studies: Facilitates the transfer of membrane proteins from detergent micelles into lipid nanodiscs or proteoliposomes.

Basic Information

Product Name	Dodecyl β-D-Cellobioside
CAS No.	74513-19-2
Molecular Formula	C24H44O11
Molecular Weight	508.60 g/mol
Synonyms	n-Dodecyl-β-D-cellobioside; Dodecyl β-D-Cellobioside; β-Dodecyl Cellobioside; Lauryl β-D-Cellobioside; n-Dodecyl-β-D-glucopyranosyl-(1→4)-β-D-glucopyranoside; DBC; Detergent β-D-Cellobioside Dodecyl; Anagrade Dodecyl-β-D-Maltoside (common alternative class)
EINECS	Contact for details

Quality Control

Our Dodecyl β-D-Cellobioside is manufactured under strict quality systems to ensure batch-to-batch consistency and high performance in sensitive research applications. Each lot undergoes rigorous analytical testing, including HPLC for purity and critical micelle concentration (CMC) verification. Certificates of Analysis (COA) with detailed specifications are provided to guarantee compliance with research-grade standards.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to prevent condensation and moisture uptake.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥ 98.0%
Critical Micelle Concentration (CMC)	0.17 - 0.21 mM (in water at 25°C)
Water Content (KF)	≤ 2.0 %
Residue on Ignition	≤ 0.1 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.