Description

Sephadex IEF is a high-performance, beaded separation medium specifically designed for isoelectric focusing (IEF) applications. This product is critical for the high-resolution separation and purification of proteins, peptides, and other ampholytes based on their isoelectric points. It is an essential tool for researchers and process developers in the fields of proteomics, biomarker discovery, and biopharmaceutical development. The compound is registered under CAS No. 73989-67-0.

Application

• High-Resolution Protein Separation: Primary use in analytical and preparative isoelectric focusing for separating complex protein mixtures.
• Proteomics Research: Essential for 2D gel electrophoresis first-dimension separation, enabling detailed protein expression analysis.
• Biopharmaceutical Purification: Used in downstream processing to isolate and purify therapeutic proteins and antibodies based on charge heterogeneity.
• Biomarker Discovery: Facilitates the identification of disease-specific protein isoforms in clinical research samples.
• Peptide Mapping: Separation of peptide fragments for structural analysis and quality control of synthetic peptides.
• Enzyme Purification: Isolation of enzymes from crude biological extracts for biochemical studies and industrial applications.

Basic Information

Item	Details
Product Name	Sephadex IEF
CAS No.	73989-67-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Sephadex IEF Gel; Sephadex IEF Medium; Dextran Cross-linked with Epichlorohydrin, IEF Grade; Isoelectric Focusing Sephadex; Polydextran Gel for IEF; Gel Filtration Medium for IEF; 2-D Electrophoresis Gel; Ampholyte Carrier Gel
EINECS	Contact for details

Quality Control

Our Sephadex IEF is manufactured under a rigorous quality management system. Each batch is tested to ensure consistent performance characteristics critical for isoelectric focusing, including bead size distribution, swelling factor, and absence of contaminants. Certificates of Analysis (COA) detailing lot-specific data are available upon request to support your research and quality documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its hygroscopic nature, the container must be kept sealed to prevent moisture uptake, which can affect the swelling properties and performance of the gel.

Specification

Item	Specification
Appearance	White to off-white free-flowing beads
Particle Size Range	40 - 120 μm (dry bead diameter)
Swelling Factor (in water)	5 - 7 mL/g
Exclusion Limit	Contact for details
pH Stability Range	2 - 11
Conductivity	Low (suitable for IEF)
Endotoxin Level	< 0.25 EU/mL (for specific grades)
Heavy Metals (as Pb)	< 10 ppm

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.