Description

D-Luciferin Methyl Ester is a chemically modified, cell-permeable analog of D-luciferin, a critical substrate for firefly luciferase. This compound is essential for enabling highly sensitive, real-time bioluminescent imaging in live cells and animals, overcoming the permeability limitations of the native substrate. It is primarily utilized by researchers and developers in the pharmaceutical, biotechnology, and contract research organization (CRO) sectors for applications in drug discovery, gene expression studies, and in vivo monitoring of biological processes.

Application

• Bioluminescent Imaging (BLI): As a superior substrate for intracellular luciferase, enabling non-invasive, real-time tracking of tumor growth, metastasis, and treatment response in live animal models.
• High-Throughput Screening (HTS): Used in cell-based assays for drug discovery to monitor gene expression, protein-protein interactions, and promoter activity with high sensitivity and low background.
• Reporter Gene Assays: Facilitates the study of transcriptional regulation and signal transduction pathways in live cells without the need for cell lysis.
• Bacterial & Microbial Detection: Employed in assays designed for rapid detection and quantification of microbial contamination or engineered bacterial strains.
• ATP-independent Luciferase Systems: Serves as a key reagent for novel luciferase-luciferin pairs engineered for improved stability, wavelength, or application-specific performance.

Basic Information

Product Name	D-Luciferin Methyl Ester
CAS No.	73918-26-0
Molecular Formula	C12H11N3O3S2
Molecular Weight	309.36 g/mol
Synonyms	D-Luciferin methyl ester; D-Luciferin O-methyl ester; Methyl D-luciferin; Firefly luciferin methyl ester; 4,5-Dihydro-2-(6-hydroxy-2-benzothiazolyl)-4-thiazolecarboxylic acid methyl ester; 2-(6-Hydroxy-2-benzothiazolyl)-4,5-dihydro-4-thiazolecarboxylic acid methyl ester; Luciferin ME
EINECS	Contact for details

Quality Control

Our D-Luciferin Methyl Ester is manufactured under strict quality management systems. Each batch undergoes comprehensive analytical testing, including HPLC for purity, NMR and MS for structural confirmation, and functional activity testing in bioluminescence assays. A detailed Certificate of Analysis (COA) is provided with every shipment, ensuring traceability and compliance with research-grade specifications.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. The product is hygroscopic (moisture-sensitive) and light-sensitive. For long-term storage, keep under inert atmosphere. Allow the vial to equilibrate to room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	Yellow to orange powder
Identification (HPLC)	Conforms to reference standard
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 1.0%
Bioluminescence Activity	≥ 90% relative to reference standard

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.