Description

2-(9H-Fluoren-9-Ylmethoxycarbonylamino)-3-Hydroxy-Butanoic Acid is a specialized Fmoc-protected amino acid derivative, a critical building block in modern solid-phase peptide synthesis (SPPS). Its primary value lies in enabling the controlled and efficient assembly of complex peptides by providing a protected hydroxyl group on the threonine side chain, which is essential for synthesizing peptides containing phosphorylated or glycosylated threonine residues. This high-purity intermediate is indispensable for research institutions and pharmaceutical companies developing therapeutic peptides, vaccines, and diagnostic agents, where precise molecular construction is paramount.

Application

• Solid-Phase Peptide Synthesis (SPPS): A key protected amino acid for the automated synthesis of complex peptide sequences.
• Pharmaceutical R&D: Used in the research and development of novel peptide-based therapeutics, including hormone analogs and enzyme inhibitors.
• Glycopeptide & Phosphopeptide Synthesis: Essential for constructing peptides where the threonine residue requires site-specific modification, such as glycosylation or phosphorylation.
• Combinatorial Chemistry Libraries: Serves as a building block for creating diverse peptide libraries used in drug discovery and screening.
• Bioconjugation: Can be utilized in the preparation of peptide-protein or peptide-small molecule conjugates for targeted therapies.
• Academic & Institutional Research: A fundamental reagent for studies in biochemistry, molecular biology, and chemical biology focused on protein function and mimicry.

Basic Information

Product Name	2-(9H-Fluoren-9-Ylmethoxycarbonylamino)-3-Hydroxy-Butanoic Acid
CAS No.	73731-37-0
Molecular Formula	C19H19NO5
Molecular Weight	341.36 g/mol
Synonyms	Fmoc-Thr-OH; Fmoc-L-Threonine; Fmoc-Threonine; N-[(9H-Fluoren-9-ylmethoxy)carbonyl]-L-threonine; (2S,3R)-2-[[(9H-Fluoren-9-ylmethoxy)carbonyl]amino]-3-hydroxybutanoic acid; Nα-Fmoc-L-threonine; N-Fmoc-threonine; Fmoc-L-Thr-OH
EINECS	Contact for details

Quality Control

Our 2-(9H-Fluoren-9-Ylmethoxycarbonylamino)-3-Hydroxy-Butanoic Acid is manufactured under strict quality management systems to ensure batch-to-batch consistency and high purity, suitable for sensitive applications like peptide synthesis. Each lot undergoes comprehensive analytical testing, including HPLC for purity, chiral analysis to confirm stereochemical integrity, and identity confirmation via spectroscopic methods. A detailed Certificate of Analysis (COA) is provided with every shipment, documenting all critical quality attributes.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This compound is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Assay (HPLC)	≥98.0%
Optical Rotation [α]D20	-15.0° to -18.0° (c=1 in DMF)
Specific Optical Rotation	Contact for details
Water Content (KF)	≤1.0%
Heavy Metals	≤20 ppm
Residue on Ignition	≤0.2%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.