Description

Formyl-Methenyl-Methylenetetrahydrofolate Synthetase is a critical enzyme involved in the folate-mediated one-carbon metabolism pathway. This compound is essential for research into nucleotide biosynthesis, amino acid metabolism, and cellular methylation processes. It is primarily utilized by biochemists, pharmaceutical researchers, and academic institutions engaged in metabolic studies, drug discovery, and enzymology.

Application

• Biochemical Research: Used as a key reagent in studies of folate metabolism and one-carbon transfer mechanisms.
• Enzyme Kinetics & Inhibition Studies: Serves as a substrate or target for investigating enzyme function and screening for potential inhibitors.
• Pharmaceutical Development: Employed in R&D for metabolic disorder therapeutics and anticancer agents targeting nucleotide synthesis.
• Diagnostic Kit Manufacturing: A component in the development of enzymatic assays for clinical diagnostics.
• Academic & Teaching Laboratories: Used for educational purposes in advanced biochemistry and molecular biology courses.
• Metabolic Pathway Analysis: Essential for in vitro reconstruction and analysis of interconnected metabolic networks.

Basic Information

Product Name	Formyl-Methenyl-Methylenetetrahydrofolate Synthetase
CAS No.	73699-18-0
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Formyl-methenyl-methylenetetrahydrofolate synthetase; 5,10-Methenyltetrahydrofolate 5-formyltetrahydrofolate cycloligase; FTHFS; EC 6.3.3.2; 5-Formyltetrahydrofolate cyclo-ligase; 5,10-Methenyltetrahydrofolate synthetase; Formyltetrahydrofolate synthetase (misnomer)
EINECS	Contact for details

Quality Control

Our Formyl-Methenyl-Methylenetetrahydrofolate Synthetase is produced and handled under stringent quality management protocols. Each batch undergoes rigorous analytical testing to ensure high purity, specific activity, and functional integrity. Certificates of Analysis (COA) detailing purity, activity units, and stability data are available upon request to support your research and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive) and should be handled under anhydrous conditions to maintain activity. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Specific Activity	Contact for details
Protein Content (Bradford)	Contact for details
pH (in solution)	6.5 - 7.5
Endotoxin Level (LAL)	< 1.0 EU/mg
Residual Moisture (KF)	< 5.0 %

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.