Description

Erythrosine Isothiocyanate is a reactive derivative of the xanthene dye Erythrosine, specifically functionalized with an isothiocyanate group. This compound is primarily valued as a critical labeling reagent for the covalent conjugation of biomolecules, enabling fluorescence-based detection and analysis. It is essential for researchers and manufacturers in the fields of bioconjugation, diagnostics, and life science research, where precise fluorescent tagging of proteins, antibodies, and other polymers is required.

Application

• Fluorescent Labeling of Biomolecules: Covalent attachment to primary amines in proteins, antibodies, peptides, and oligonucleotides for fluorescence microscopy, flow cytometry, and immunoassays.
• Diagnostic Kit Development: Used as a key component in the manufacture of fluorescent probes and reagents for in vitro diagnostic (IVD) applications.
• Bioconjugation Research: Serves as a model compound or active reagent in studies focused on developing new conjugation chemistries and biomaterial modifications.
• Cellular and Tissue Staining: Preparation of fluorescent conjugates for histology, cytology, and live-cell imaging studies.
• Sensor and Assay Development: Integration into biosensors and assay platforms where a stable, bright fluorescent signal is required.
• Polymer and Material Science: Modification of synthetic polymers or surfaces to introduce fluorescent properties for tracking or functional studies.

Basic Information

Product Name	Erythrosine Isothiocyanate
CAS No.	73676-43-4
Molecular Formula	C20H6I4Na2O5·NCS (commonly cited) / Contact for details
Molecular Weight	Contact for details
Synonyms	Erythrosine ITC; Erythrosine B Isothiocyanate; Acid Red 51 Isothiocyanate; FD&C Red No. 3 Isothiocyanate; 2',4',5',7'-Tetraiodo-3',6'-dihydroxyspiro[isobenzofuran-1(3H),9'-[9H]xanthen]-3-one Isothiocyanate; 2',4',5',7'-Tetraiodofluorescein Isothiocyanate; Reactive Red Dye
EINECS	Contact for details

Quality Control

Our Erythrosine Isothiocyanate is produced under controlled conditions to ensure high reactivity and labeling efficiency. Each batch undergoes rigorous analytical testing, including HPLC for purity, spectroscopic identification (IR/UV-Vis), and functional activity testing. We provide comprehensive Certificates of Analysis (COA) with each shipment to guarantee traceability and compliance with your research or production specifications.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C or as indicated on the label. This material is hygroscopic (moisture-sensitive); ensure the container is sealed under inert atmosphere or with desiccant after opening to maintain stability and prevent hydrolysis of the reactive isothiocyanate group.

Specification

Item	Specification
Appearance	Dark red to brownish-red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 90% (Area %)
Functional Activity (Labeling)	Conforms to reference
Water Content (KF)	≤ 5.0 %
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.