Description

Cytochrome P455 CAS NO 73468-77-6 is a specialized cytochrome P450 enzyme variant, a critical hemoprotein involved in the oxidative metabolism of a vast array of endogenous and exogenous compounds. Its primary value lies in enabling essential biochemical research into drug metabolism, toxicology, and steroid biosynthesis pathways. This product is indispensable for pharmaceutical R&D laboratories, academic research institutions, and biotechnology companies focused on metabolic studies, enzyme kinetics, and inhibitor screening.

Application

• Drug Metabolism and Interaction Studies: Used as a key reagent in in vitro systems to assess the metabolic stability of new chemical entities and potential drug-drug interactions.
• Enzyme Kinetics and Characterization: Essential for determining kinetic parameters (Km, Vmax) and studying the catalytic mechanism of cytochrome P450 enzymes.
• Toxicological Screening: Employed to investigate the bioactivation of procarcinogens and the formation of reactive metabolites that may cause toxicity.
• Biochemical Research: Serves as a standard or substrate in studies of steroid hormone biosynthesis, fatty acid metabolism, and other P450-mediated pathways.
• Inhibitor Discovery and Validation: A critical tool for screening and characterizing novel compounds that inhibit or induce specific P450 isoforms.
• Educational and Diagnostic Use: Utilized in advanced teaching laboratories and in the development of diagnostic assays related to metabolic function.

Basic Information

Item	Details
Product Name	Cytochrome P455
CAS No.	73468-77-6
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cytochrome P-455; P455; CYP455; Hemoprotein P455; Microsomal Cytochrome P455; Liver Microsomal Cytochrome P455; Oxidoreductase, P455; Cytochrome P450 455
EINECS	Contact for details

Quality Control

Our Cytochrome P455 is produced and handled under stringent quality protocols to ensure high purity, specific activity, and batch-to-batch consistency, which are critical for reliable research data. Each lot undergoes comprehensive analytical testing, including spectral purity analysis (Soret band), specific activity assays, and protein content determination. A detailed Certificate of Analysis (COA) documenting these specifications is provided with every shipment to support your quality assurance and regulatory documentation needs.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below for long-term stability. This material is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use to prevent absorption of atmospheric moisture. For short-term use, thaw on ice and keep cold. Avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Dark red to brown, lyophilized powder or solution
Purity (Spectral)	> 90% (A417/A280 ratio)
Specific Activity	Contact for details (nmol/min/mg protein)
Protein Content	Determined by Bradford or Lowry method
Solubility	Soluble in appropriate buffer (e.g., potassium phosphate, Tris-HCl)
Identification (UV-Vis Spectroscopy)	Characteristic Soret band ~417 nm (reduced CO difference spectrum)
Endotoxin	< 1.0 EU/mg (for cell-based applications)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.