Description

Calmodulin is a ubiquitous, calcium-binding messenger protein that plays a critical role in a vast array of cellular signaling pathways. Its ability to regulate numerous enzymes, including kinases, phosphatases, and cyclic nucleotide phosphodiesterases, makes it an essential component for fundamental biological processes. This high-purity Calmodulin CAS NO 73298-54-1 is indispensable for research and development in biochemistry, molecular biology, and pharmaceutical discovery, enabling the study of calcium-mediated signal transduction.

Application

Calmodulin is a key reagent in life science research and diagnostic development. Its primary applications include:

• Biochemical Research: Used as a critical cofactor in enzymatic assays to study calcium-dependent activation of proteins like calcineurin, nitric oxide synthase, and adenylate cyclase.
• Drug Discovery & Screening: Employed in high-throughput screening (HTS) platforms to identify novel modulators of calmodulin-dependent pathways for therapeutic intervention.
• Diagnostic Kit Manufacturing: Serves as a core component in ELISA and other immunoassays designed to detect calmodulin-binding proteins or autoantibodies.
• Structural Biology: Essential for X-ray crystallography and NMR studies to elucidate the mechanisms of calcium-induced conformational changes and protein-protein interactions.
• Cell Signaling Studies: Utilized in vitro to reconstitute signaling cascades and investigate the role of calcium/calmodulin in processes such as muscle contraction, neurotransmission, and cell cycle progression.
• Educational & Reference Standards: Provided as a purified protein standard for university laboratories and quality control in related bioproduct manufacturing.

Basic Information

Product Name	Calmodulin
CAS No.	73298-54-1
Molecular Formula	Contact for details
Molecular Weight	~16.7 kDa (approx., varies by species and post-translational modifications)
Synonyms	CAM; Calmodulin 1; CALM1; CaM; Calcium-Modulated Protein; Phosphodiesterase Activator; Troponin C-like Protein; Calmodulin (Bovine Brain); Calmodulin (Recombinant); Ca2+-Calmodulin
EINECS	Contact for details

Quality Control

Our Calmodulin is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and functional activity. We adhere to relevant guidelines for research-grade biologicals, ensuring the product meets the exacting standards required for sensitive experimental applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized powder at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C to -80°C to prevent repeated freeze-thaw cycles. The product is hygroscopic (moisture-sensitive); ensure the container is sealed tightly after each use in a low-humidity environment.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~17 kDa
Purity (HPLC)	≥ 95%
Protein Content (Biuret)	≥ 90%
Biological Activity	Activates Calmodulin-Dependent Phosphodiesterase
Endotoxin Level (LAL)	< 1.0 EU/mg
Solubility	Soluble in water or buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.