Description

Erythrosin B Isothiocyanate, Isomer Ii is a reactive derivative of the xanthene dye Erythrosin B, specifically functionalized with an isothiocyanate group for covalent conjugation. This compound matters for researchers and manufacturers requiring a highly specific, fluorescent labeling reagent for proteins, antibodies, and other biomolecules. It is primarily needed by professionals in the biotechnology, diagnostics, and life sciences sectors for applications in fluorescence microscopy, flow cytometry, and immunoassay development.

Application

• Fluorescent Labeling of Proteins and Antibodies: Covalent conjugation via the isothiocyanate group to amine residues for creating fluorescent probes.
• Immunofluorescence and Cell Staining: Used as a tag in immunohistochemistry (IHC) and immunocytochemistry (ICC) for visualizing cellular components.
• Flow Cytometry Reagent: Preparation of fluorescently-labeled ligands or antibodies for cell sorting and analysis.
• Biomolecular Tracking: Conjugation to peptides, oligonucleotides, or other biomolecules to study their localization and interactions.
• Diagnostic Assay Development: A key component in the formulation of fluorescent detection systems for clinical and research diagnostics.
• Material Science Research: Potential use in labeling polymers or nanoparticles for tracking in composite materials.

Basic Information

Product Name	Erythrosin B Isothiocyanate, Isomer Ii
CAS No.	72814-84-7
Molecular Formula	C27H19I4N3O6S
Molecular Weight	1083.07 g/mol
Synonyms	Erythrosin B ITC; Erythrosin B Isothiocyanate Isomer II; Erythrosin B Isothiocyanate; Acid Red 51 Isothiocyanate; 2-(6-Hydroxy-2,4,5,7-tetraiodo-3-oxo-3H-xanthen-9-yl)benzoic acid isothiocyanate; FD&C Red No. 3 Isothiocyanate; CI 45430 Isothiocyanate; Reactive Red Dye
EINECS	Contact for details

Quality Control

Our Erythrosin B Isothiocyanate, Isomer Ii is produced under strict quality management protocols. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. A comprehensive Certificate of Analysis (COA) detailing purity, performance in conjugation, and absence of critical impurities is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to moisture, heat, or light will degrade its reactivity. Keep the container sealed under an inert atmosphere such as argon or nitrogen for long-term storage to maintain optimal performance.

Specification

Item	Specification
Appearance	Dark red to brownish-red powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0% (Isomer II)
Loss on Drying	≤ 2.0 %
Conjugation Efficiency	Tested and reported per batch
Solubility	Soluble in DMF, DMSO; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.