Description

Listeriolysin CAS NO 72270-41-8 is a potent, pore-forming cholesterol-dependent cytolysin (CDC) exotoxin produced by the bacterium *Listeria monocytogenes*. This protein is a critical virulence factor, essential for the pathogen's ability to escape the phagosome and replicate within the host cell cytoplasm. It is a vital reagent for researchers in microbiology, immunology, and infectious disease, enabling the study of bacterial pathogenesis, host-pathogen interactions, and the development of novel therapeutic and diagnostic strategies.

Application

• Microbiological Research: Fundamental studies on the mechanism of action, structure, and genetics of *Listeria monocytogenes* virulence.
• Immunology & Vaccine Development: Used as an antigen to study host immune responses and in the research and development of vaccines against listeriosis.
• Cell Biology Studies: A tool to permeabilize cellular membranes in a controlled manner for studying intracellular processes and protein trafficking.
• Diagnostic Reagent Production: Serves as a key component in the development and validation of immunoassays and PCR-based diagnostic kits for detecting *Listeria* infection.
• Toxicology & Pharmacology: Research into cytolysin inhibitors and novel antimicrobial agents targeting pore-forming toxins.
• Biochemical Standard: Acts as a purified protein standard for analytical methods such as SDS-PAGE, Western Blot, and activity assays.

Basic Information

Product Name	Listeriolysin
CAS No.	72270-41-8
Molecular Formula	Contact for details
Molecular Weight	Approx. 58-60 kDa (monomer)
Synonyms	Listeriolysin O; LLO; Hemolysin; Cholesterol-dependent cytolysin; *Listeria* cytolysin; Pore-forming toxin; Listeria hemolysin; CDC family toxin; Listeria monocytogenes hemolysin; Listeria toxin
EINECS	Contact for details

Quality Control

Our Listeriolysin is produced and purified under stringent conditions to ensure high biological activity and purity, suitable for research applications. Each batch is subjected to rigorous quality testing, including activity assays (hemolytic assay), purity analysis by SDS-PAGE, and sterility testing where applicable. Certificates of Analysis (COA) detailing specific purity, activity (in HU/mg or similar units), and analytical data are provided with every shipment to guarantee traceability and consistency for your critical research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store lyophilized material at -20°C or below. Reconstituted solutions should be aliquoted and stored at -70°C or below to prevent repeated freeze-thaw cycles and activity loss. The product is hygroscopic (moisture-sensitive); ensure containers are sealed tightly after use.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (SDS-PAGE)	Single band at ~58-60 kDa under reducing conditions
Purity (HPLC)	≥ 90%
Protein Content (BCA/Bradford)	≥ 95% of total mass
Biological Activity (Hemolytic Assay)	≥ 1 x 10⁵ HU/mg protein
Endotoxin Level (LAL)	< 1.0 EU/μg
Solubility	Soluble in aqueous buffers (e.g., PBS)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.