Description

Succinimidyl 6-(Biotinamido)Hexanoate is a versatile and high-purity NHS ester derivative of biotin, designed for efficient biotinylation of primary amines under mild aqueous conditions. This compound is critical for researchers and manufacturers requiring stable, long-chain spacer arms to minimize steric hindrance in sensitive detection and capture applications. It is an essential reagent for the bioconjugation workflows of pharmaceutical developers, diagnostic kit manufacturers, and academic research institutions focused on proteomics, immunoassays, and affinity purification.

Application

• Biotinylation of antibodies, proteins, peptides, and oligonucleotides for use in ELISA, Western blotting, and flow cytometry.
• Preparation of affinity matrices and biosensors by conjugating biotin to surfaces or polymers for streptavidin/avidin capture systems.
• Labeling of cellular components and drug candidates for target identification and pull-down assays in drug discovery.
• Manufacturing of in-vitro diagnostic (IVD) components and lateral flow assay conjugates.
• Development of targeted therapeutic conjugates and advanced drug delivery systems.
• Site-specific protein labeling and immobilization for structural biology and microarray technologies.

Basic Information

Product Name	Succinimidyl 6-(Biotinamido)Hexanoate
CAS No.	72040-63-2
Molecular Formula	C20H30N4O6S
Molecular Weight	454.54 g/mol
Synonyms	NHS-LC-Biotin; Biotinamidohexanoic Acid N-Hydroxysuccinimide Ester; Biotin-X NHS Ester; 6-((6-(Biotinamido)hexanoyl)amino)hexanoic Acid N-Succinimidyl Ester; N-Succinimidyl 6-(Biotinamido)Hexanoate; EZ-Link™ NHS-LC-Biotin (Trade Name); Sulfo-NHS-LC-Biotin (related sulfonated form); N-Hydroxysuccinimide ester of biotin-XX
EINECS	Contact for details

Quality Control

Our Succinimidyl 6-(Biotinamido)Hexanoate is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation by NMR and mass spectrometry, to ensure it meets the high standards required for research and diagnostic applications. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store desiccated at -20°C or below. This product is hygroscopic (moisture-sensitive). Allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and hydrolysis.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Assay	≥ 90.0% (NHS ester content)
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; slightly soluble in water (hydrolyzes)

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.