Description

Monobromobimane is a highly reactive, cell-permeable fluorescent thiol-specific alkylating agent. Its core value lies in its ability to form stable, intensely fluorescent adducts with thiol groups, enabling sensitive detection and quantification in complex biological matrices. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors who require precise tools for studying protein structure, redox states, and glutathione levels.

Application

• Fluorescent Labeling of Thiol Groups in proteins, peptides, and other biomolecules for analytical detection.
• Quantification of Glutathione (GSH) and other low-molecular-weight thiols in cellular extracts and biological fluids.
• Analysis of Protein Structure and Folding by probing the accessibility and reactivity of cysteine residues.
• Monitoring Cellular Redox Status and oxidative stress in research applications.
• Derivatization Agent in HPLC and Capillary Electrophoresis for enhanced sensitivity in separation sciences.
• Proteomics and Biomarker Discovery workflows requiring specific thiol tagging.

Basic Information

Product Name	Monobromobimane
CAS No.	71418-44-5
Molecular Formula	C10H11BrN2O2
Molecular Weight	271.11 g/mol
Synonyms	mBBr; Bimane, monobromo-; (2-Bromoethyl)trimethylammonium bimane; Thiolite™ MB; 3-(Bromomethyl)-2,5,6-trimethyl-1H,7H-pyrazolo[1,2-a]pyrazole-1,7-dione; SynaptoGreen™ C4
EINECS	Contact for details

Quality Control

Our Monobromobimane is manufactured under strict quality control protocols to ensure high purity and batch-to-batch consistency, suitable for sensitive research and analytical applications. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles. We adhere to relevant industry standards, and custom purity grades, including HPLC-grade and research-grade, are available to meet specific project requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 2-8°C (refrigerated) or as indicated on the label. This product is hygroscopic (moisture-sensitive) and light-sensitive; desiccants are recommended for long-term storage. Keep container tightly sealed when not in use to minimize exposure to atmospheric moisture.

Specification

Item	Specification
Appearance	White to off-white crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95%
Loss on Drying	≤ 2.0 %
Solubility	Soluble in DMSO, Acetonitrile, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.