Description

o-Nitrophenyl β-D-Cellobioside is a chromogenic substrate specifically designed for the detection and quantification of cellulase enzyme activity. This compound matters because it provides a highly sensitive and convenient colorimetric assay method, enabling precise measurement of enzymatic hydrolysis in research and industrial quality control settings. It is primarily needed by biotechnology and life science research laboratories, as well as industrial manufacturers developing or monitoring enzymatic processes for biofuel production, textile processing, and pulp and paper applications.

Application

• Cellulase Enzyme Assays: Primary substrate for the spectrophotometric determination of endo-1,4-β-glucanase (cellulase) activity in research and diagnostic kits.
• Biomass Conversion Research: Used to screen and characterize novel cellulolytic enzymes for the efficient breakdown of lignocellulosic biomass into fermentable sugars.
• Industrial Process Monitoring: Employed in quality control laboratories within biofuel, textile, detergent, and food processing industries to monitor the activity and stability of commercial cellulase preparations.
• Microbiological Studies: Aids in the identification and study of cellulase-producing microorganisms, including bacteria and fungi.
• Enzyme Kinetics Studies: Serves as a model substrate for investigating the catalytic mechanism, inhibition, and optimal conditions of β-glucosidase and cellulase enzymes.
• High-Throughput Screening (HTS): Suitable for automated assay systems in pharmaceutical and biotechnology sectors for discovering new enzyme inhibitors or activators.

Basic Information

Product Name	o-Nitrophenyl β-D-Cellobioside
CAS No.	70867-33-3
Molecular Formula	C18H25NO13
Molecular Weight	463.39 g/mol
Synonyms	4-Nitrophenyl β-D-cellobioside; 2-Nitrophenyl β-cellobioside; ONPC; o-Nitrophenyl cellobioside; β-ONP-Cellobioside; 1-(o-Nitrophenyl)-β-D-cellobioside; Cellobiose, 1-(2-nitrophenyl)-, β-D-; 2-Nitrophenyl β-D-glucopyranosyl-(1→4)-β-D-glucopyranoside
EINECS	Contact for details

Quality Control

Our o-Nitrophenyl β-D-Cellobioside is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity verification and identity confirmation by spectroscopic methods, to ensure it meets the high standards required for sensitive enzymatic research. A comprehensive Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment to guarantee reproducible and reliable performance in your assays.

Storage

Preserve in a tightly closed container, protected from light. Store at 2-8°C in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed container to reach room temperature before opening to minimize moisture condensation.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Melting Point	Contact for details
Specific Rotation	Contact for details
Solubility	Soluble in water, DMSO, DMF

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.