Description

1-Palmitoyl-2-(14-Proxylstearoyl)Glycero-3-Phospho-n-(4-Azido-3-Nitrophenyl)Ethanolamine is a specialized, spin-labeled phospholipid derivative designed for advanced biophysical and biochemical research. This compound matters for its unique dual-functionality, incorporating a nitroxide spin label (proxyl) for electron paramagnetic resonance (EPR) spectroscopy and a photoactivatable aryl azide group for covalent cross-linking studies. It is primarily needed by research scientists and product developers in the life sciences sector, particularly those investigating membrane dynamics, protein-lipid interactions, and the development of novel diagnostic or therapeutic agents.

Application

• Membrane Biophysics Research: As a spin-labeled phospholipid analog for probing lipid bilayer properties, phase transitions, and membrane fluidity using EPR spectroscopy.
• Protein-Lipid Interaction Studies: To investigate the binding, orientation, and dynamics of membrane-associated proteins through site-directed spin labeling and cross-linking techniques.
• Liposome and Model Membrane Engineering: Incorporation into liposomal formulations to create traceable, functionalized membranes for drug delivery system development.
• Structural Biology: Aiding in the determination of membrane protein structures and conformational changes by providing distance constraints via EPR.
• Diagnostic Probe Development: Serving as a key intermediate or active component in the synthesis of advanced contrast agents or molecular probes for imaging.
• Photocrosslinking Experiments: Utilizing the aryl azide moiety for UV-induced covalent capture of transient molecular interactions within biological membranes.

Basic Information

Product Name	1-Palmitoyl-2-(14-Proxylstearoyl)Glycero-3-Phospho-n-(4-Azido-3-Nitrophenyl)Ethanolamine
CAS No.	70764-33-9
Molecular Formula	C₄₈H₈₄N₄O₁₀P
Molecular Weight	925.17 g/mol
Synonyms	1-Palmitoyl-2-(14-Proxylstearoyl)-sn-glycero-3-phosphoethanolamine-N-(4-azido-2-nitrophenyl); Proxyl-PE-ANP; Spin-labeled PE-ANP; Nitroxide-labeled phosphatidylethanolamine azide; 14-Proxyl PE ANP; 1-Palmitoyl-2-stearoyl(14-proxyl)-sn-glycero-3-phosphoethanolamine-N-(4-azido-3-nitrophenyl); ANP-PE (proxyl labeled); EPR-active phospholipid crosslinker
EINECS	Contact for details

Quality Control

Our products undergo rigorous quality testing to ensure compliance with the high-purity standards required for sensitive research applications. Each batch is characterized using advanced analytical techniques including NMR, mass spectrometry, and HPLC to confirm identity, purity, and functional group integrity. Certificates of Analysis (COA) detailing specific batch results are available upon request. We adhere to a quality management system to ensure traceability and consistency for our research-grade biochemicals.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store desiccated at -20°C or below under an inert atmosphere (argon or nitrogen). This product is light-sensitive (store away from light), easily oxidized (store under inert atmosphere), and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. Aliquot contents upon receipt to avoid repeated freeze-thaw cycles and exposure to air.

Specification

Item	Specification
Appearance	Yellow to orange solid or film
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Assay (EPR)	Active spin label content ≥ 90%
Solubility	Soluble in chloroform, DMSO; forms vesicles in aqueous buffer

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.