Description

Pepstatyl, Glu- is a synthetic, cell-permeable peptide aldehyde inhibitor specifically designed to target aspartic proteases—including pepsin, cathepsin D, and renin—with high potency and selectivity. It serves as a critical research tool for mechanistic enzymology, lysosomal function studies, and therapeutic target validation in metabolic and neurodegenerative disease models. This compound is widely utilized by pharmaceutical R&D laboratories, academic biochemistry departments, and contract research organizations engaged in protease-targeted drug discovery and pathway analysis.

Application

• In vitro inhibition of aspartic proteases (e.g., pepsin, cathepsin D, renin) for enzyme kinetics and structural biology studies
• Lysosomal activity modulation in autophagy and apoptosis research using cultured mammalian cells
• Controlled protease suppression in primary neuron and macrophage models to investigate inflammatory and neurodegenerative pathways
• Reference standard in HPLC and LC-MS method development for protease inhibitor quantification
• Tool compound in preclinical target engagement assays for aspartic protease-targeted therapeutics
• Component of custom assay kits for high-throughput screening of protease inhibitors

Basic Information

Product Name	Pepstatyl, Glu-
CAS No.	70706-87-5
Molecular Formula	C34H59N7O10
Molecular Weight	733.89 g/mol
Synonyms	Pepstatin A Glu; Pepstatin A, glutamic acid derivative; (3S,4S)-4-amino-3-hydroxy-6-methylheptanoic acid-(3S,4S)-4-amino-3-hydroxy-6-methylheptanoic acid-L-glutamic acid; L-Glutamic acid, N-[(3S,4S)-4-amino-3-hydroxy-6-methylheptanoyl]-N-[(3S,4S)-4-amino-3-hydroxy-6-methylheptanoyl]-; Glutamyl-pepstatin; Pepstatyl-Glu; Glu-Pepstatin; Pepstatin Glu conjugate
EINECS	Not listed

Quality Control

Our products undergo rigorous quality testing to ensure compliance with industry standards. Certificates of Analysis (COA) are available upon request. All batches of Pepstatyl, Glu- are verified for identity (IR, NMR, MS), assay (HPLC), and purity (>95% by HPLC), consistent with requirements for research-grade biochemical reagents. Testing adheres to ISO 9001–certified laboratory protocols and supports compliance with GLP and GMP-aligned research practices.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C under inert atmosphere (argon or nitrogen) to prevent degradation. Due to its hygroscopic nature, minimize exposure to ambient humidity; allow vial to equilibrate to room temperature in a desiccated environment before opening.

Specification

Item	Specification
Appearance	White to off-white lyophilized powder
Identification (IR)	Conforms to reference spectrum
Identification (HPLC)	Retention time matches reference standard
Assay (HPLC)	≥95.0%
Related Substances (HPLC)	Single impurity ≤1.0%; Total impurities ≤3.0%
Water Content (Karl Fischer)	≤5.0%
Residue on Ignition	≤0.1%
Solubility	Soluble in DMSO (≥10 mg/mL); sparingly soluble in water, methanol

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.