Description

Hemoglobin Indianapolis is a specific, well-characterized variant of hemoglobin, a critical oxygen-transporting metalloprotein in red blood cells. This high-purity biochemical is essential for advanced research into hemoglobinopathies, oxygen-binding kinetics, and the development of diagnostic standards. It serves as a vital reference material for clinical laboratories, pharmaceutical R&D teams, and academic institutions focused on hematology and blood substitute technologies. The compound is supplied with full traceability and rigorous quality documentation to ensure experimental reproducibility and reliability.

Application

• Biochemical Research: Used as a reference standard in studies of hemoglobin structure, function, and genetic mutations.
• Clinical Diagnostics: Serves as a calibrant or control material in assays for abnormal hemoglobin detection and quantification.
• Pharmaceutical Development: Employed in R&D for blood substitutes and oxygen-therapeutic agents.
• Hematology Studies: Critical for investigating oxygen affinity, cooperativity, and the pathophysiology of related blood disorders.
• Educational Reference: Used in advanced university teaching laboratories for protein chemistry and medical biochemistry.

Basic Information

Product Name	Hemoglobin Indianapolis
CAS No.	70699-68-2
Molecular Formula	C2952H4664N812O840S8Fe4
Molecular Weight	~64,500 Da (for tetramer)
Synonyms	Hb Indianapolis; Hemoglobin variant Indianapolis; α-2 globin chain variant; Unstable hemoglobin Indianapolis; Hemoglobin α2112(G19)His→Arg β2; HBA2: c.338A>G (p.His113Arg); Contact for additional identifiers.
EINECS	Contact for details

Quality Control

Our Hemoglobin Indianapolis is produced and handled under controlled conditions to ensure the highest standards of purity and identity. Each batch is subjected to comprehensive analytical testing, including electrophoresis, HPLC for purity assessment, and spectroscopic verification. A detailed Certificate of Analysis (COA) is provided, documenting the specification, test methods, and results for parameters such as identity, purity, and stability. Our quality system is designed to meet the stringent requirements of research and diagnostic applications.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below. This product is hygroscopic (moisture-sensitive); allow the sealed vial to equilibrate to room temperature before opening to prevent condensation. Aliquot into working portions to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder or solution
Identification (Electrophoresis)	Conforms to standard for Hb Indianapolis
Identification (Spectroscopy)	Characteristic Soret band ~410-415 nm
Purity (HPLC)	≥ 95% (main peak)
Protein Content	Determined by UV-Vis or Bradford assay
Solvent/Buffer	Specified per grade (e.g., PBS, H2O)
Biological Activity (O2 binding)	Assessed by oxygen dissociation curve

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.