Description

Dansylalanyllysine Chloromethyl Ketone is a specialized synthetic peptide derivative designed as an active site-directed irreversible inhibitor for serine proteases. This compound matters for researchers and developers requiring precise biochemical tools to study, modulate, or inhibit specific enzymatic pathways. It is primarily needed by professionals in pharmaceutical R&D, proteomics research, and biochemical assay development for investigating enzyme mechanisms and developing therapeutic agents.

Application

• Protease Inhibition Studies: Used as an active site titrant and irreversible inhibitor for characterizing serine proteases like trypsin, plasmin, and thrombin.
• Biochemical Research: A critical tool in enzymology for probing catalytic mechanisms, active site geometry, and enzyme kinetics.
• Pharmaceutical Development: Serves as a key intermediate or reference standard in the development of novel protease-targeting therapeutics.
• Diagnostic Assay Development: Employed in the design and calibration of sensitive fluorometric assays leveraging its dansyl fluorophore for detection.
• Proteomics & Protein Chemistry: Facilitates the study of protein-protein interactions and the functional annotation of protease families.

Basic Information

Product Name	Dansylalanyllysine Chloromethyl Ketone
CAS No.	70592-17-5
Molecular Formula	C26H40ClN5O5S
Molecular Weight	578.14 g/mol
Synonyms	DALCK; Dansyl-Ala-Lys-CH2Cl; Nε-Dansyl-L-lysyl-Nα-(chloroacetyl)-L-alaninamide; 5-(Dimethylamino)naphthalene-1-sulfonyl-alanyl-lysine chloromethyl ketone; L-Alaninamide, N2-(chloroacetyl)-N6-[5-(dimethylamino)-1-naphthalenesulfonyl]-L-lysyl-; Protease Inhibitor DALCK; TLCK Dansyl Derivative
EINECS	Contact for details

Quality Control

Our Dansylalanyllysine Chloromethyl Ketone is manufactured under controlled conditions to ensure high purity and batch-to-batch consistency. Each lot undergoes rigorous analytical testing, including HPLC for purity assessment and spectroscopic methods for structural confirmation. Certificates of Analysis (COA) detailing purity, identity, and impurity profiles are available upon request to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below in a dry environment. This product is hygroscopic (moisture-sensitive) and light-sensitive; prolonged exposure to ambient conditions or moisture should be avoided to maintain stability and potency.

Specification

Item	Specification
Appearance	Yellow to light brown powder
Identification (HPLC)	Conforms
Identification (MS)	Conforms
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.