Description

H-His-Pna CAS NO 70324-65-1 is a specialized peptide derivative, specifically a histidine-containing para-nitroanilide substrate. This compound is valued for its role as a precise biochemical tool in enzymatic research and development. It is primarily utilized by research institutions and pharmaceutical companies engaged in protease and peptidase activity studies, high-throughput screening, and biochemical assay development.

Application

• Protease/Peptidase Substrate: Used as a chromogenic substrate for the kinetic study and characterization of various serine and cysteine proteases.
• High-Throughput Screening (HTS): Employed in drug discovery platforms to identify and evaluate potential enzyme inhibitors.
• Biochemical Assay Development: Serves as a key component in developing sensitive and specific colorimetric assays for enzymatic activity.
• Enzyme Mechanism Studies: Facilitates research into enzyme kinetics, specificity, and catalytic mechanisms due to its cleavable para-nitroanilide group.
• Diagnostic Reagent Intermediate: Can be used in the synthesis of reagents for clinical diagnostic tests targeting specific proteolytic enzymes.

Basic Information

Product Name	H-His-Pna
CAS No.	70324-65-1
Molecular Formula	C16H20N6O4
Molecular Weight	360.37 g/mol
Synonyms	Histidine p-nitroanilide; L-Histidyl-p-nitroanilide; H-His-pNA; His-pNA; N-α-Benzoyl-L-histidine p-nitroanilide; Bz-His-pNA; Protease Substrate H-His-pNA; Bz-L-His-pNA
EINECS	Contact for details

Quality Control

Our H-His-Pna is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity verification and identity confirmation via spectroscopic methods. We provide full traceability and Certificates of Analysis (COA) are available for all shipments to ensure compliance with your research and development requirements.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term storage, consider desiccated conditions.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 95.0%
Water Content (KF)	≤ 5.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.