Description

Pertussis Toxin is a critical virulence factor and exotoxin produced by the bacterium *Bordetella pertussis*, the causative agent of whooping cough. This biologically active protein is an essential tool for biomedical research, particularly in the study of G-protein coupled receptor (GPCR) signaling pathways due to its unique ability to ADP-ribosylate the alpha subunit of inhibitory G proteins (Gi). It is primarily utilized by pharmaceutical R&D laboratories, immunology researchers, and vaccine manufacturers for mechanistic studies, assay development, and as a reference standard.

Application

• Biomedical Research: Fundamental tool for studying G-protein signaling, cell biology, and immune modulation.
• Vaccine Development & Quality Control: Used as a challenge agent, reference standard, and for potency testing in acellular pertussis (aP) vaccine production and lot release.
• Immunology Studies: Investigates the role of T-cell responses, lymphocyte trafficking, and the pathogenesis of *Bordetella pertussis* infection.
• Assay Development: Serves as a key component in functional cell-based assays (e.g., cAMP accumulation assays) to screen for GPCR activity.
• Neuroscience Research: Used to study the effects on neuronal signaling and nociception.
• Diagnostic Reagent Production: May be employed in the development of immunological tests and diagnostic kits.

Basic Information

Product Name	Pertussis Toxin
CAS No.	70323-44-3
Molecular Formula	Contact for details
Molecular Weight	Approx. 105,000 Da (Hexameric A-B structure)
Synonyms	Pertussis Toxin; PTX; Islet-Activating Protein; IAP; Lymphocytosis-Promoting Factor; LPF; Pertussigen; *Bordetella pertussis* Toxin; Whooping Cough Toxin
EINECS	Contact for details

Quality Control

Our Pertussis Toxin is produced and tested under stringent conditions to ensure high biological activity and purity, suitable for research and industrial applications. Each batch is accompanied by a comprehensive Certificate of Analysis (COA) detailing purity, protein concentration, biological activity (e.g., CHO cell clustering assay), and absence of significant contaminants. We adhere to relevant quality management principles to support our clients' regulatory and compliance needs.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store frozen at -20°C or below. Avoid repeated freeze-thaw cycles. This material is hygroscopic (moisture-sensitive) and should be handled in a controlled environment to maintain stability. Aliquotting upon receipt is recommended for frequent use.

Specification

Item	Specification
Appearance	White or off-white lyophilized powder
Identification (SDS-PAGE)	Characteristic band pattern corresponding to S1-S5 subunits
Protein Content (UV)	>95%
Purity (HPLC)	>90%
Biological Activity	Defined by CHO cell clustering or ADP-ribosyltransferase activity; specific potency per batch on COA
Endotoxin Level (LAL)	< 1.0 EU/µg
Solubility	Soluble in aqueous buffers
Moisture Content (KF)	< 5.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.