Description

Cathepsin N is a lysosomal cysteine protease belonging to the papain superfamily, playing a significant role in intracellular protein turnover and specific extracellular matrix remodeling. Its precise enzymatic activity makes it a valuable tool for biochemical research and a target for therapeutic development in conditions involving dysregulated proteolysis. This high-purity enzyme is essential for researchers and developers in the pharmaceutical, biotechnology, and diagnostic sectors, facilitating studies in cancer biology, inflammation, and lysosomal storage disorders. Cathepsin N CAS NO 70132-10-4 is supplied with detailed activity data to ensure experimental reproducibility.

Application

• Biomedical Research: Used as a specific protease in enzymatic assays to study its role in cellular processes, including apoptosis, autophagy, and antigen presentation.
• Drug Discovery & Screening: Serves as a critical target enzyme for the identification and validation of novel inhibitors for therapeutic applications in arthritis, osteoporosis, and metastatic cancer.
• Diagnostic Development: Employed in the development of fluorogenic or colorimetric substrate-based assays for detecting and quantifying Cathepsin N activity in biological samples.
• Protein Biochemistry: Utilized for the controlled digestion of proteins and peptides in structural studies or to generate specific fragments for analysis.
• Cell Culture & Tissue Engineering: Applied in research models to investigate extracellular matrix degradation and tissue remodeling mechanisms.

Basic Information

Product Name	Cathepsin N
CAS No.	70132-10-4
Molecular Formula	Contact for details
Molecular Weight	Contact for details
Synonyms	Cath N; CTSN; Cathepsin N (Bos taurus); Lysosomal cysteine protease N; EC 3.4.22.-
EINECS	Contact for details

Quality Control

Our Cathepsin N is produced and tested under stringent quality management systems to ensure batch-to-batch consistency and high biological activity. Each lot is accompanied by a comprehensive Certificate of Analysis (COA) detailing key parameters such as specific activity, purity, and absence of contaminating proteases. We adhere to relevant guidelines for research-grade enzymes, ensuring the material is suitable for sensitive in vitro applications. COAs are available upon request and can be provided to support regulatory submissions for pre-clinical research.

Storage

Preserve in a tightly closed container, protected from light. For long-term stability, store at -20°C or below in a non-frost-free freezer. The product is hygroscopic (moisture-sensitive); allow the vial to equilibrate to room temperature in a desiccator before opening to prevent condensation and moisture uptake. Aliquot reconstituted material to avoid repeated freeze-thaw cycles.

Specification

Item	Specification
Appearance	Lyophilized powder
Identification (Activity)	Conforms
Specific Activity	≥ [Value] units/mg protein
Purity (SDS-PAGE)	≥ 90%
Protein Content (Bradford)	Reported per batch
Solubility	Fully soluble in appropriate assay buffer
Endotoxin Level	< 1.0 EU/μg

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.