Description

Int Formazan CAS NO 7781-49-9 is a high-purity chemical compound widely recognized as a key intermediate and analytical reagent. Its primary value lies in its role as a chromogenic substrate in vital biochemical assays, particularly for detecting and quantifying cellular metabolic activity. This product is essential for research and quality control laboratories in the pharmaceutical, biotechnology, and life sciences sectors, where precise and reliable results are critical.

Application

• Cell Viability and Proliferation Assays: Used as a substrate in MTT and related formazan-based assays to measure mitochondrial activity in live cells.
• Biochemical Research: Serves as a critical reagent in enzymatic studies and metabolic pathway analysis.
• Pharmaceutical Development: Employed in high-throughput screening (HTS) for drug discovery and cytotoxicity testing of new compounds.
• Diagnostic Kits: A key component in commercial diagnostic kits for clinical and research applications.
• Microbiology: Used in assays to study microbial growth and metabolic function.
• Material Science: Investigated for potential use in developing sensors and colorimetric indicators.

Basic Information

Product Name	Int Formazan
CAS No.	7781-49-9
Molecular Formula	C₁₈H₁₂N₄O₄
Molecular Weight	348.31 g/mol
Synonyms	INT Formazan; Iodonitrotetrazolium Formazan; 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazol-3-ium; 2-(p-Iodophenyl)-3-(p-nitrophenyl)-5-phenyltetrazolium chloride formazan; INT; Iodonitrotetrazolium Violet; INT-Violet; INT Formazan salt
EINECS	Contact for details

Quality Control

Our Int Formazan is manufactured under strict quality management systems to ensure batch-to-batch consistency and high performance in sensitive applications. Each lot undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via IR spectroscopy. A detailed Certificate of Analysis (COA) documenting purity, assay, and related substance profiles is provided with every shipment to support your quality assurance protocols.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). Due to its light-sensitive nature and tendency to be easily oxidized, prolonged exposure to light and air should be avoided. For long-term stability, consider storage under an inert atmosphere.

Specification

Item	Specification
Appearance	Dark violet to purple crystalline powder
Identification (IR)	Conforms to reference spectrum
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 1.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.