Description

Pentanoic Acid, 4-Methyl-4-[(5-Nitro-2-Pyridinyl)Dithio]-, 2,5-Dioxo-3-Sulfo-1-Pyrrolidinyl Ester is a specialized heterocyclic organic compound featuring a cleavable disulfide linker and an activated ester group. This molecular architecture makes it a critical reagent for bioconjugation and chemical biology research, enabling the controlled attachment of molecules to proteins, peptides, and other biomolecules. It is primarily needed by pharmaceutical R&D teams, diagnostic kit manufacturers, and academic research institutions developing targeted therapeutics and advanced analytical assays.

Application

• Bioconjugation Reagent: For site-specific labeling of proteins, antibodies, and peptides via reaction with primary amines (lysine residues).
• Proteomics Research: Used in the synthesis of activity-based probes (ABPs) and affinity tags for studying enzyme function and protein interactions.
• Diagnostic Assay Development: Conjugation of enzymes, fluorophores, or haptens to antibodies for ELISA, lateral flow, and other immunoassay formats.
• Drug Delivery System Fabrication: Building block for creating cleavable linkers in antibody-drug conjugates (ADCs) and other targeted delivery platforms.
• Surface Functionalization: Modification of nanoparticles, biosensors, and chromatographic media with bioactive ligands.
• Crosslinking Agent: For introducing reversible disulfide bonds between molecules to study dynamics or create responsive materials.

Basic Information

Product Name	Pentanoic Acid, 4-Methyl-4-[(5-Nitro-2-Pyridinyl)Dithio]-, 2,5-Dioxo-3-Sulfo-1-Pyrrolidinyl Ester
CAS No.	663599-00-6
Molecular Formula	C15H15N3O9S3
Molecular Weight	485.48 g/mol
Synonyms	4-Methyl-4-((5-nitropyridin-2-yl)disulfanyl)pentanoic acid 2,5-dioxopyrrolidin-1-yl 3-sulfopropanoate; 5-Nitro-2-pyridyl disulfide-activated NHS ester (sulfonated); SPDP analogue (sulfonated); Sulfo-LC-SPDP; Heterobifunctional crosslinker (amine-reactive, disulfide); N-Succinimidyl 3-((2-(5-nitro-2-pyridyldithio)ethyl)carbamoyl)propionate-1-sulfonate; Activated ester disulfide reagent
EINECS	Contact for details

Quality Control

Our production of this high-purity biochemical reagent adheres to strict in-process controls to ensure batch-to-batch consistency and optimal reactivity. Every lot is characterized by HPLC, NMR, and mass spectrometry to confirm structural identity and purity. A comprehensive Certificate of Analysis (COA) detailing purity, functional performance, and impurity profiles is provided with each shipment to support your quality and regulatory documentation.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under desiccant. This compound is hygroscopic (moisture-sensitive) and light-sensitive. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture uptake. For long-term stability, store under inert atmosphere.

Specification

Item	Specification
Appearance	Yellow to orange powder or crystalline solid
Identification (IR)	Conforms to structure
Identification (NMR)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water
Functional Activity	Conjugation efficiency confirmed by model reaction

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.