Description

Benzamide, n-[2-[2-(2-Aminoethoxy)Ethoxy]Ethyl]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]- is a specialized, high-purity photoaffinity labeling reagent containing a trifluoromethyl-diazirine group. This compound is critical for advanced biochemical research, enabling the covalent cross-linking and identification of protein-ligand interactions and binding sites. It is primarily utilized by research scientists and process developers in pharmaceutical R&D, proteomics, and chemical biology for target identification and validation studies.

Application

• Photoaffinity Labeling (PAL): A key reagent for irreversible photo-crosslinking in the study of biomolecular interactions, including protein-protein, protein-nucleic acid, and protein-small molecule binding.
• Chemical Proteomics: Used in activity-based protein profiling (ABPP) to identify and characterize enzyme active sites and drug targets within complex biological systems.
• Pharmaceutical R&D: Enables the mapping of drug binding sites and the elucidation of mechanisms of action for novel therapeutic candidates.
• Bioconjugation & Probe Synthesis: Serves as a versatile building block for synthesizing advanced molecular probes and affinity tags due to its reactive amine and photoreactive diazirine functionalities.
• Structural Biology: Assists in the structural analysis of transient or weak biological complexes by creating stable covalent bonds upon UV irradiation.

Basic Information

Item	Details
Product Name	Benzamide, n-[2-[2-(2-Aminoethoxy)Ethoxy]Ethyl]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]-
CAS No.	655224-73-0
Molecular Formula	C₁₆H₂₁F₃N₄O₃
Molecular Weight	374.36 g/mol
Synonyms	4-[3-(Trifluoromethyl)-3H-diazirin-3-yl]-N-[2-[2-(2-aminoethoxy)ethoxy]ethyl]benzamide; 3-(4-[[2-[2-(2-Aminoethoxy)ethoxy]ethyl]carbamoyl]phenyl)-3-(trifluoromethyl)diaziridine; TPD-Benzamide-PEG3-amine; Photoaffinity Labeling Reagent Benzamide-PEG3-Amine; Trifluoromethylphenyl Diazirine Benzamide Derivative; PAL Probe Benzamide; Benzamide-PEG3-NH2 TPD; Contact for details on additional trade names.
EINECS	Contact for details

Quality Control

Every batch of Benzamide, n-[2-[2-(2-Aminoethoxy)Ethoxy]Ethyl]-4-[3-(Trifluoromethyl)-3H-Diazirin-3-Yl]- is manufactured and analyzed under strict quality management systems to ensure the high purity and consistency required for sensitive research applications. Our products undergo rigorous quality testing, including NMR, HPLC, and MS, to ensure compliance with exacting research-grade standards. A comprehensive Certificate of Analysis (COA) detailing purity, identity, and impurity profiles is provided with each shipment.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) in a dry, freezer. This compound is strictly light-sensitive (protect from all light exposure) and hygroscopic (moisture-sensitive). Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture ingress.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (NMR)	Conforms to structure
Identification (MS)	Conforms to structure
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.