Description

5-Bromo-6-Chloro-3-Indoxyl Choline Phosphate is a specialized chromogenic substrate used in enzymatic detection systems. This compound matters for its high sensitivity and specificity in biochemical assays, particularly for detecting phosphatase activity. It is primarily needed by research institutions, diagnostic kit manufacturers, and pharmaceutical companies developing advanced detection methodologies.

Application

• Enzymatic Substrate for Phosphatases: Key component in histochemical and cytochemical staining kits for locating and quantifying alkaline and acid phosphatase activity in tissue samples.
• Diagnostic Reagent Manufacturing: Used in the formulation of clinical diagnostic kits for detecting enzyme markers associated with various diseases and physiological conditions.
• Biochemical Research: Employed in laboratory research to study enzyme kinetics, inhibition, and cellular signaling pathways involving choline-containing compounds and phosphatases.
• Microbiology and Cell Biology: Applied in assays to identify bacterial species or to monitor gene expression and reporter systems in molecular biology.
• Pharmaceutical Development: Utilized in high-throughput screening (HTS) assays during drug discovery to identify potential therapeutic compounds that modulate phosphatase activity.

Basic Information

Product Name	5-Bromo-6-Chloro-3-Indoxyl Choline Phosphate
CAS No.	641571-93-9
Molecular Formula	C13H17BrClN2O5P
Molecular Weight	427.62 g/mol
Synonyms	BCIP-Choline Phosphate; 5-Bromo-6-chloro-3-indolyl phosphorylcholine; 3-Indolylphosphorylcholine, 5-bromo-6-chloro-; Choline phosphate 5-bromo-6-chloro-3-indoxyl ester; BCIP-ChP; Phosphorylcholine 5-bromo-6-chloro-3-indoxyl ester
EINECS	Contact for details

Quality Control

Our 5-Bromo-6-Chloro-3-Indoxyl Choline Phosphate is produced under strict quality management protocols. Each batch undergoes comprehensive analytical testing, including HPLC for purity and identity confirmation via spectroscopic methods, to ensure it meets the stringent requirements for research and diagnostic applications. A Certificate of Analysis (COA) detailing purity, assay, and impurity profiles is provided with every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This product is hygroscopic (moisture-sensitive) and should be handled in a dry environment to prevent degradation. For long-term stability, consider storage under inert atmosphere or desiccant.

Specification

Item	Specification
Appearance	White to off-white powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content (KF)	≤ 2.0%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.