Description

Phosphine-Biotin CAS NO 608514-42-7 is a specialized bioconjugation reagent that combines a biotin moiety with a phosphine group, enabling highly efficient and selective labeling of biomolecules. This compound is critical for researchers and manufacturers requiring site-specific modification of proteins, nucleic acids, and other biological targets under mild, aqueous conditions. It is primarily utilized in the development of advanced diagnostics, targeted therapeutics, and sophisticated proteomics research platforms within the pharmaceutical and biotechnology sectors.

Application

• Staudinger Ligation in Bioconjugation: Enables chemoselective labeling of azide-tagged biomolecules for the creation of antibody-drug conjugates (ADCs) and other targeted biotherapeutics.
• Proteomics and Biomarker Discovery: Used for the selective capture and enrichment of azide-modified proteins or glycoproteins for mass spectrometry analysis.
• Diagnostic Assay Development: Facilitates the site-specific attachment of biotin to probes and antibodies for use in ELISA, lateral flow assays, and other diagnostic platforms.
• Cell Surface Engineering: Allows for the covalent tagging of cell surface azides with biotin for imaging, tracking, and sorting applications in cell biology.
• Materials Science Functionalization: Employed to introduce biotin handles onto azide-functionalized nanoparticles, polymers, and surfaces for creating bioactive materials.
• Chemical Biology Tool Development: Serves as a key reagent in toolkit development for studying protein function, interactions, and localization.

Basic Information

Product Name	Phosphine-Biotin
CAS No.	608514-42-7
Molecular Formula	C19H38N4O3PS
Molecular Weight	424.56 g/mol
Synonyms	Biotin-PEG3-Phosphine; Biotin Phosphine; Phosphino-PEG3-Biotin; (2-(2-(2-(5-((3aS,4S,6aR)-2-Oxohexahydro-1H-thieno[3,4-d]imidazol-4-yl)pentanamido)ethoxy)ethoxy)ethyl)(diphenylphosphino); Biotinylated Phosphine; Staudinger-Biotin Reagent; Biotin-PEG3-TPP; PEG3-Biotin-Phosphine
EINECS	Contact for details

Quality Control

Every batch of Phosphine-Biotin is manufactured and tested under strict quality management systems to ensure high purity and consistent performance for sensitive bioconjugation applications. Our products undergo rigorous quality testing, including HPLC for purity and NMR for structural confirmation, to ensure compliance with research and development standards. Certificates of Analysis (COA) detailing lot-specific results are available upon request.

Storage

Preserve in a tightly closed container, protected from light. Store at -20°C or below under an inert atmosphere (e.g., argon or nitrogen) to prevent oxidation. Allow the sealed vial to equilibrate to room temperature before opening to minimize moisture condensation. For long-term stability, store desiccated.

Specification

Item	Specification
Appearance	White to off-white solid
Identification (HPLC)	Conforms
Identification (NMR)	Conforms
Purity (HPLC)	≥ 95%
Water Content (KF)	≤ 1.0%

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.