Description

Naphthol As Phosphate Disodium Salt is a key chromogenic substrate used in enzymatic detection systems. This compound is critical for producing a visible, colored precipitate in biochemical assays, enabling precise visualization and quantification. It is primarily required by manufacturers and research laboratories in the life sciences, diagnostics, and biotechnology sectors for applications such as immunohistochemistry (IHC), in situ hybridization (ISH), and ELISA.

Application

• Immunohistochemistry (IHC): As a substrate for alkaline phosphatase (AP) to localize specific antigens in tissue sections.
• In Situ Hybridization (ISH): For the enzymatic detection of nucleic acid sequences in cells and tissues.
• Enzyme-Linked Immunosorbent Assay (ELISA): Used in colorimetric detection for quantitative immunoassays.
• Western Blotting: For the chromogenic detection of proteins transferred to membranes.
• Diagnostic Kit Manufacturing: A core component in clinical diagnostic kits for various diseases.
• Research & Development: In biochemical and histological research for signal amplification and visualization.
• Cell Biology Studies: For staining and imaging applications in cellular analysis.

Basic Information

Product Name	Naphthol As Phosphate Disodium Salt
CAS No.	69815-54-9
Molecular Formula	C20H13NNa2O6P
Molecular Weight	464.27 g/mol
Synonyms	Naphthol AS-PO4 Disodium Salt; Naphthol AS Phosphate, Disodium Salt; 3-Hydroxy-2-naphthoic acid 2,4-dimethylanilide phosphate disodium salt; Naphthol AS-MX Phosphate Disodium Salt; Fast Red TR Salt substrate; Alkaline Phosphatase Substrate; AS-PO4; AS-MX Phosphate
EINECS	Contact for details

Quality Control

Our Naphthol As Phosphate Disodium Salt is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for diagnostic and research applications. Certificates of Analysis (COA) with detailed specifications are provided for every shipment.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at 15-25°C. This material is hygroscopic (moisture-sensitive) and light-sensitive; ensure the container is kept tightly sealed in a desiccated environment to maintain stability and performance.

Specification

Item	Specification
Appearance	Off-white to light yellow powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Water Content	≤ 2.0%
Solubility	Soluble in water, DMSO
Functional Performance	Suitable for IHC/ISH applications

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.