Description

2,3-Bis(4-Nitrophenyl)-5-Phenyltetrazolium Chloride is a vital tetrazolium salt widely used as a redox indicator and chromogenic substrate in biochemical assays. Its core value lies in its ability to form a highly colored, water-insoluble formazan dye upon reduction, providing a sensitive and quantifiable signal. This compound is essential for researchers and quality control laboratories in the pharmaceutical, biotechnology, and life science sectors, particularly for applications like cell viability testing (MTT assay), enzyme activity assays, and microbiological analysis.

Application

• Cell Viability and Proliferation Assays (MTT Assay): A critical reagent for quantifying mitochondrial activity in live cells, widely used in drug discovery, cytotoxicity testing, and cancer research.
• Enzyme Activity Detection: Serves as an electron acceptor for dehydrogenase enzymes (e.g., succinate dehydrogenase), enabling the colorimetric measurement of enzymatic activity in biochemical studies.
• Microbiological Testing: Used as an indicator in microbial viability tests and for the detection of microbial contamination in pharmaceutical and cosmetic products.
• Histochemistry and Staining: Employed in tissue staining to localize sites of dehydrogenase enzyme activity, aiding in diagnostic pathology and research.
• Biochemical Research Reagent: A fundamental tool for studying redox reactions, electron transport chains, and cellular metabolism in vitro.
• Quality Control in Manufacturing: Applied in sterility testing and cell-based potency assays for biopharmaceuticals and other advanced therapy medicinal products (ATMPs).

Basic Information

Product Name	2,3-Bis(4-Nitrophenyl)-5-Phenyltetrazolium Chloride
CAS No.	69231-13-6
Molecular Formula	C25H17ClN6O4
Molecular Weight	500.90 g/mol
Synonyms	INT; p-Iodonitrotetrazolium Violet; 2-(4-Iodophenyl)-3-(4-nitrophenyl)-5-phenyl-2H-tetrazolium Chloride; Iodonitrotetrazolium Chloride; INT Chloride; 2,3-Bis(p-nitrophenyl)-5-phenyltetrazolium Chloride; 2,3-Di(4-nitrophenyl)-5-phenyltetrazolium Chloride; Tetrazolium Violet
EINECS	Contact for details

Quality Control

Our 2,3-Bis(4-Nitrophenyl)-5-Phenyltetrazolium Chloride is manufactured under strict quality management systems. Each batch undergoes rigorous analytical testing, including HPLC for purity and identity confirmation, to ensure it meets the high standards required for sensitive biochemical applications. Certificates of Analysis (COA) detailing purity, assay, and impurity profiles are available upon request to support your regulatory and research needs.

Storage

Preserve in a tightly closed container, protected from light. Store in a cool, dry place at a controlled room temperature (15-25°C). This product is hygroscopic (moisture-sensitive); ensure the container is sealed after each use to prevent degradation. Keep away from incompatible materials.

Specification

Item	Specification
Appearance	Yellow to orange crystalline powder
Identification (IR)	Conforms to structure
Purity (HPLC)	≥ 98.0%
Loss on Drying	≤ 2.0%
Residue on Ignition	≤ 0.5%
Solubility	Soluble in DMSO, DMF; sparingly soluble in water

Note: Specifications can be tailored. Please contact us for the detailed technical data sheet of a specific grade.